RRC ID 28556
Author Ota S, Matsuda T, Takeshita T, Yamazaki T, Kazama Y, Abe T, Kawano S.
Title Phenotypic spectrum of Parachlorella kessleri (Chlorophyta) mutants produced by heavy-ion irradiation.
Journal Bioresour Technol
Abstract Heavy-ion mutagenesis is a technology used for effective production of genetic mutants. This study demonstrates that algal breeding using a unicellular alga, Parachlorella kessleri, by heavy-ion mutagenesis can improve lipid yield in laboratory experiments. The primary screening yielded 23 mutants among which a secondary screening yielded 7 strains, which were subjected to phenotypic assays. P. kessleri strains produced by heavy-ion radiation spanned a broad spectrum of phenotypes that differed in lipid content and fatty acid profiles. Starch grain morphology was distinctively altered in one of the mutants. The growth of strain PK4 was comparable to that of the wild type under stress-free culture conditions, and the mutant also produced large quantities of lipids, a combination of traits that may be of commercial interest. Thus, heavy-ion irradiation is an effective mutagenic agent for microalgae and may have potential in the production of strains with gain-of-function phenotypes.
Volume 149
Pages 432-8
Published 2013-12-1
DOI 10.1016/j.biortech.2013.09.079
PII S0960-8524(13)01511-3
PMID 24135567
MeSH Argon / chemistry Biomass Carbon / chemistry Chlorophyta / genetics* Chlorophyta / growth & development Chlorophyta / radiation effects Fatty Acids / metabolism Gas Chromatography-Mass Spectrometry Genetic Testing Heavy Ions* Mutagenesis / radiation effects Mutation / genetics* Phenotype Starch / chemistry Starch / metabolism Time Factors
IF 7.539
Times Cited 14
Algae NIES-2152