RRC ID 28650
Author Matsuyama A, Arai R, Yashiroda Y, Shirai A, Kamata A, Sekido S, Kobayashi Y, Hashimoto A, Hamamoto M, Hiraoka Y, Horinouchi S, Yoshida M.
Title ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Journal Nat Biotechnol
Abstract Cloning of the entire set of an organism's protein-coding open reading frames (ORFs), or 'ORFeome', is a means of connecting the genome to downstream 'omics' applications. Here we report a proteome-scale study of the fission yeast Schizosaccharomyces pombe based on cloning of the ORFeome. Taking advantage of a recombination-based cloning system, we obtained 4,910 ORFs in a form that is readily usable in various analyses. First, we evaluated ORF prediction in the fission yeast genome project by expressing each ORF tagged at the 3' terminus. Next, we determined the localization of 4,431 proteins, corresponding to approximately 90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein. Furthermore, using leptomycin B, an inhibitor of the nuclear export protein Crm1, we identified 285 proteins whose localization is regulated by Crm1.
Volume 24(7)
Pages 841-7
Published 2006-7-1
DOI 10.1038/nbt1222
PII nbt1222
PMID 16823372
MeSH Antifungal Agents / pharmacology Fatty Acids, Unsaturated / pharmacology Gene Expression Regulation, Fungal / drug effects* Gene Library* Gene Order / genetics* Genes, Fungal Internet Karyopherins / antagonists & inhibitors Karyopherins / genetics Luminescent Proteins / genetics Open Reading Frames / genetics* Proteomics / methods Receptors, Cytoplasmic and Nuclear / antagonists & inhibitors Receptors, Cytoplasmic and Nuclear / genetics Saccharomyces cerevisiae Proteins / genetics Schizosaccharomyces / genetics* Schizosaccharomyces pombe Proteins / genetics*
IF 36.553
Times Cited 406
DNA material pARG1K-FFH1c (RDB06437) S. pombe ORFeome Clone