Reference - Detail
| RRC ID | 28655 |
|---|---|
| Author | Shibuya T, Watanabe K, Yamashita H, Shimizu K, Miyashita H, Abe M, Moriya T, Ohta H, Sonoda H, Shimosegawa T, Tabayashi K, Sato Y. |
| Title | Isolation and characterization of vasohibin-2 as a homologue of VEGF-inducible endothelium-derived angiogenesis inhibitor vasohibin. |
| Journal | Arterioscler Thromb Vasc Biol |
| Abstract |
OBJECTIVE:We recently isolated vasohibin, a novel vascular endothelial growth factor (VEGF)-inducible endothelium-derived angiogenesis inhibitor. Our aim is to find DNA sequences homologous to vasohibin and determine their expression profile. METHODS AND RESULTS:By the search of DNA sequences in the database, we found one homologous gene and designated it vasohibin-2. Overall amino acid sequence homology between the prototype vasohibin (vasohibin-1) and vasohibin-2 was >50%. Vasohibin-2 exhibited antiangiogenic activity. Vasohibin-2 expression in cultured endothelial cells was low and not inducible by the stimulation that induced vasohibin-1. However, the immunohistochemical analysis revealed that vasohibin-1 and -2 were diffusely expressed in endothelial cells in embryonic organs during mid-gestation. After that time point, vasohibin-1 and -2 became faint, but persisted to a certain extent in arterial endothelial cells from late gestation to neonate. Expression of vasohibin-1 and -2 could be augmented in vivo by local transfection with the VEGF gene in the embryonic brain or by cutaneous wounding in adult mice. CONCLUSIONS:These results suggest that vasohibin-2, in combination with vasohibin-1, forms a novel family of angiogenesis inhibitors. |
| Volume | 26(5) |
| Pages | 1051-7 |
| Published | 2006-5-1 |
| DOI | 10.1161/01.ATV.0000216747.66660.26 |
| PII | 01.ATV.0000216747.66660.26 |
| PMID | 16528006 |
| MeSH | Angiogenesis Inhibitors / analysis Angiogenesis Inhibitors / genetics* Angiogenesis Inhibitors / pharmacology Animals Cells, Cultured Endothelial Cells / chemistry Humans Immunohistochemistry Mice Neovascularization, Physiologic / drug effects Organ Culture Techniques RNA, Messenger / analysis Reverse Transcriptase Polymerase Chain Reaction |
| IF | 6.604 |
| Times Cited | 66 |
| WOS Category | HEMATOLOGY PERIPHERAL VASCULAR DISEASE |
| Resource | |
| DNA material | p3XFLAG-CMV-14-hVASH2 (RDB08519) |