Reference - Detail
| RRC ID | 28670 |
|---|---|
| Author | Tachibana M, Ueda J, Fukuda M, Takeda N, Ohta T, Iwanari H, Sakihama T, Kodama T, Hamakubo T, Shinkai Y. |
| Title | Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9. |
| Journal | Genes Dev |
| Abstract |
Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic mark for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential for murine embryogenesis. There is a single G9a-related methyltransferase in mammals, called GLP/Eu-HMTase1. Here we show that GLP is also important for H3-K9 methylation of mouse euchromatin. GLP-deficiency led to embryonic lethality, a severe reduction of H3-K9 mono- and dimethylation, the induction of Mage-a gene expression, and HP1 relocalization in embryonic stem cells, all of which were phenotypes of G9a-deficiency. Furthermore, we show that G9a and GLP formed a stoichiometric heteromeric complex in a wide variety of cell types. Biochemical analyses revealed that formation of the G9a/GLP complex was dependent on their enzymatic SET domains. Taken together, our new findings revealed that G9a and GLP cooperatively exert H3-K9 methyltransferase function in vivo, likely through the formation of higher-order heteromeric complexes. |
| Volume | 19(7) |
| Pages | 815-26 |
| Published | 2005-4-1 |
| DOI | 10.1101/gad.1284005 |
| PII | gad.1284005 |
| PMID | 15774718 |
| PMC | PMC1074319 |
| MeSH | Animals Euchromatin / enzymology* Histone Methyltransferases Histone-Lysine N-Methyltransferase / chemistry* Histone-Lysine N-Methyltransferase / metabolism Histones / metabolism* Lysine / metabolism* Methylation Mice Protein Methyltransferases Protein Structure, Quaternary Stem Cells / enzymology |
| IF | 9.527 |
| Times Cited | 493 |
| WOS Category | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | 14-1 (RDB05729) |