RRC ID 28695
Author Sakudoh T, Kuwazaki S, Iizuka T, Narukawa J, Yamamoto K, Uchino K, Sezutsu H, Banno Y, Tsuchida K.
Title CD36 homolog divergence is responsible for the selectivity of carotenoid species migration to the silk gland of the silkworm Bombyx mori.
Journal J Lipid Res
Abstract Dietary carotenoids are absorbed in the intestine and delivered to various tissues by circulating lipoproteins; however, the mechanism underlying selective delivery of different carotenoid species to individual tissues remains elusive. The products of the Yellow cocoon (C) gene and the Flesh (F) gene of the silkworm Bombyx mori determine the selectivity for transport of lutein and β-carotene, respectively, to the silk gland. We previously showed that the C gene encodes Cameo2, a CD36 family member, which is thought to function as a transmembrane lipoprotein receptor. Here, we elucidated the molecular identity of the F gene product by positional cloning, as SCRB15, a paralog of Cameo2 with 26% amino acid identity. In the F mutant, SCRB15 mRNA structure was severely disrupted, due to a 1.4 kb genomic insertion in a coding exon. Transgenic expression of SCRB15 in the middle silk gland using the binary GAL4-UAS expression system enhanced selective β-carotene uptake by the middle silk gland, while transgenic expression of Cameo2 enhanced selective lutein uptake under the same GAL4 driver. Our findings indicate that divergence of genes in the CD36 family determines the selectivity of carotenoid species uptake by silk gland tissue and that CD36-homologous proteins can discriminate among carotenoid species.
Volume 54(2)
Pages 482-95
Published 2013-2-1
DOI 10.1194/jlr.M032771
PII jlr.M032771
Description F(肉色繭)の原因遺伝子はSCRB15である。SCRB15はCD36 familyに属するリポタンパク質レセプターをコードする。W06系統(+F/+F)では、SCRB15のエクソン6に1400bpの挿入配列が存在する。
PMID 23160179
PMC PMC3588874
MeSH Amino Acid Sequence Animals Biological Transport Bombyx / genetics* Bombyx / metabolism CD36 Antigens / genetics* Carotenoids / metabolism* Chromosome Mapping Chromosomes, Insect / genetics Genetic Loci / genetics Genomics Insect Proteins / chemistry Insect Proteins / genetics* Insect Proteins / metabolism* Male Molecular Sequence Data Mutation RNA, Messenger / genetics RNA, Messenger / metabolism Sequence Homology, Nucleic Acid* Silk / metabolism* Substrate Specificity Transgenes / genetics beta Carotene / metabolism
IF 4.483
Times Cited 26
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Silkworms c44 w06 e09 b10 c05 c10 c43 c11