RRC ID 28704
Author Benghezal M, Gotthardt D, Cornillon S, Cosson P.
Title Localization of the Rh50-like protein to the contractile vacuole in Dictyostelium.
Journal Immunogenetics
Abstract The human Rhesus (Rh) family consists of three polytopic membrane proteins present at the cell surface of red blood cells. Although Rh proteins are essential for the expression of the blood group system their biological function remains unclear. In this study, the gene encoding a protein homologous to Rh50 in Dictyostelium discoideum was sequenced. The Rh50-like protein was localized to the contractile vacuole, the organelle responsible for maintenance of osmotic equilibrium within the cell. However, Rh50-like-deficient mutants in which the Rh50-like gene was disrupted did not appear to exhibit a phenotype related to osmoregulation. Nevertheless, these mutants may provide a valuable tool for studying the function of the rhesus protein.
Volume 52(3-4)
Pages 284-8
Published 2001-1-1
DOI 10.1007/s002510000279
PMID 11220631
MeSH Amino Acid Sequence Animals Blood Proteins / analysis* Blood Proteins / chemistry* Blood Proteins / genetics Blood Proteins / physiology Blotting, Western Dictyostelium / chemistry* Dictyostelium / cytology* Dictyostelium / genetics Dictyostelium / metabolism Fluorescent Antibody Technique Gene Deletion Glycoproteins / analysis* Glycoproteins / chemistry* Glycoproteins / genetics Glycoproteins / physiology Humans Membrane Glycoproteins* Molecular Sequence Data Osmotic Pressure Phenotype Sequence Alignment Sequence Homology, Amino Acid* Vacuoles / chemistry*
IF 2.621
Times Cited 40
WOS Category IMMUNOLOGY GENETICS & HEREDITY
Resource
Cellular slime molds G02286