RRC ID 28709
Author Miura S, Gan JW, Brzostowski J, Parisi MJ, Schultz CJ, Londos C, Oliver B, Kimmel AR.
Title Functional conservation for lipid storage droplet association among Perilipin, ADRP, and TIP47 (PAT)-related proteins in mammals, Drosophila, and Dictyostelium.
Journal J Biol Chem
Abstract Intracellular neutral lipid storage droplets are essential organelles of eukaryotic cells, yet little is known about the proteins at their surfaces or about the amino acid sequences that target proteins to these storage droplets. The mammalian proteins Perilipin, ADRP, and TIP47 share extensive amino acid sequence similarity, suggesting a common function. However, while Perilipin and ADRP localize exclusively to neutral lipid storage droplets, an association of TIP47 with intracellular lipid droplets has been controversial. We now show that GFP-tagged TIP47 co-localizes with isolated intracellular lipid droplets. We have also detected a close juxtaposition of TIP47 with the surfaces of lipid storage droplets using antibodies that specifically recognize TIP47, further indicating that TIP47 associates with intracellular lipid storage droplets. Finally, we show that related proteins from species as diverse as Drosophila and Dictyostelium can also target mammalian or Drosophila lipid droplet surfaces in vivo. Thus, sequence and/or structural elements within this evolutionarily ancient protein family are necessary and sufficient to direct association to heterologous intracellular lipid droplet surfaces, strongly indicating that they have a common function for lipid deposition and/or mobilization.
Volume 277(35)
Pages 32253-7
Published 2002-8-30
DOI 10.1074/jbc.M204410200
PII S0021-9258(20)70089-X
PMID 12077142
MeSH Adipose Tissue / metabolism Animals CHO Cells Carrier Proteins Cricetinae DNA-Binding Proteins / chemistry DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Dictyostelium Drosophila Green Fluorescent Proteins Humans Intracellular Signaling Peptides and Proteins* Luminescent Proteins / genetics Luminescent Proteins / metabolism Mammals Membrane Proteins / chemistry Membrane Proteins / genetics Membrane Proteins / metabolism* Mice Perilipin-1 Perilipin-2 Perilipin-3 Phosphoproteins / chemistry Phosphoproteins / genetics Phosphoproteins / metabolism* Phylogeny Pregnancy Proteins* Recombinant Fusion Proteins / metabolism Sequence Homology, Amino Acid Species Specificity Vesicular Transport Proteins
IF 4.238
Times Cited 285
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