論文 - 詳細
RRC ID | 28715 |
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著者 | Yasukawa H, Kuroita T, Tamura K, Yamaguchi K. |
タイトル | Identification of a penicillin-sensitive carboxypeptidase in the cellular slime mold Dictyostelium discoideum. |
ジャーナル | Biol Pharm Bull |
Abstract |
Penicillin binding proteins (PBPs) are penicillin-sensitive DD-peptidases catalyzing the terminal stages of bacterial cell wall assembly. We identified a Dictyostelium discoideum gene that encodes a protein of 522 amino acids showing similarity to Escherichia coli PBP4. The D. discoideum protein conserves three consensus sequences (SXXK, SXN and KTG) that are responsible for the catalytic activities of PBPs. The gene product prepared in the cell-free translation system showed carboxypeptidase activity but the activity was not detected in the presence of penicillin G. These results demonstrate that the D. discoideum gene encodes a eukaryotic form of penicillin-sensitive carboxypeptidase. |
巻・号 | 26(7) |
ページ | 1018-20 |
公開日 | 2003-7-1 |
DOI | 10.1248/bpb.26.1018 |
PMID | 12843630 |
MeSH | Amino Acid Sequence / drug effects Amino Acid Sequence / physiology Animals Base Sequence / drug effects Base Sequence / physiology Carboxypeptidases / genetics Carboxypeptidases / metabolism* Dictyosteliida / drug effects* Dictyosteliida / enzymology Dictyostelium / drug effects* Dictyostelium / enzymology Enzyme Activation / drug effects Enzyme Activation / physiology Molecular Sequence Data Penicillins / pharmacology* |
IF | 1.863 |
引用数 | 4 |
WOS 分野 | PHARMACOLOGY & PHARMACY |
リソース情報 | |
細胞性粘菌 | G02052 |