Reference - Detail
| RRC ID | 28717 |
|---|---|
| Author | El-Halawany MS, Ohkouchi S, Shibata H, Hitomi K, Maki M. |
| Title | Identification of cysteine protease inhibitors that belong to cystatin family 1 in the cellular slime mold Dictyostelium discoideum. |
| Journal | Biol Chem |
| Abstract |
Family 1 cystatins are cytosolic inhibitors of cysteine proteases, and they are conserved in higher eukaryotes. We characterized two newly identified family 1 cystatins of the cellular slime mold Dictyostelium discoideum, cystatin A1 and A2. Their recombinant proteins showed specific inhibitory activity against papain and cathepsin B, respectively. Using specific polyclonal antibodies, we found that cystatin A1 is stably expressed throughout the life cycle of Dictyostelium, whereas cystatin A2 expression is up-regulated during the course of development. |
| Volume | 385(6) |
| Pages | 547-50 |
| Published | 2004-6-1 |
| DOI | 10.1515/BC.2004.065 |
| PMID | 15255188 |
| MeSH | Amino Acid Sequence Animals Cystatins / chemistry* Cystatins / genetics Cystatins / metabolism Cysteine Proteinase Inhibitors / chemistry* Cysteine Proteinase Inhibitors / genetics Cysteine Proteinase Inhibitors / metabolism Dictyostelium / drug effects Dictyostelium / enzymology* Dictyostelium / genetics Molecular Sequence Data Protozoan Proteins / chemistry* Protozoan Proteins / genetics Protozoan Proteins / metabolism Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Sequence Homology, Amino Acid |
| IF | 3.27 |
| Times Cited | 5 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Cellular slime molds | G03158 G02091 |