Reference - Detail
RRC ID | 28729 |
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Author | Yoshino T, Maeda Y, Amagai A. |
Title | The real factor for polypeptide elongation in Dictyostelium cells is EF-2B, not EF-2A. |
Journal | Biochem Biophys Res Commun |
Abstract |
Polypeptide elongation factor 2 (EF-2) plays an essential role in protein synthesis and is believed to be indispensable for cell proliferation. Recently, it has been demonstrated that there are two kinds of EF-2 (EF-2A and EF-2B with 76.6% of sequence identity at the amino acid level) in Dictyostelium discoideum. Although the knockout of EF-2A slightly impaired cytokinesis, EF-2A null cells exhibited almost normal protein synthesis and cell growth, suggesting that there is another molecule capable of compensating for EF-2 function. Since EF-2B is the most likely candidate, we examined its function using ef-2b knockdown cells prepared by the RNAi method. Our results strongly suggest that EF-2B is required for protein synthesis and cell proliferation, functioning as the real EF-2. Interestingly, the expressions of ef-2a and ef-2b mRNAs during development are reversely regulated, and the ef-2b expression is greatly augmented in ef-2a null cells. |
Volume | 359(3) |
Pages | 586-91 |
Published | 2007-8-3 |
DOI | 10.1016/j.bbrc.2007.05.155 |
PII | S0006-291X(07)01113-8 |
PMID | 17560550 |
MeSH | Amino Acid Sequence Animals Cell Differentiation Cells, Cultured Conserved Sequence Dictyostelium / chemistry Dictyostelium / cytology Dictyostelium / genetics Dictyostelium / metabolism* Gene Expression Regulation Molecular Sequence Data Peptide Elongation Factor 2 / chemistry Peptide Elongation Factor 2 / classification Peptide Elongation Factor 2 / genetics Peptide Elongation Factor 2 / metabolism* Peptides / metabolism* RNA Interference RNA, Messenger / genetics Sequence Alignment |
IF | 2.985 |
Times Cited | 2 |
WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
Resource | |
Cellular slime molds | G00508 G01573 |