| RRC ID |
28732
|
| 著者 |
Nagayama K, Itono S, Yoshida T, Ishiguro S, Ochiai H, Ohmachi T.
|
| タイトル |
Antisense RNA inhibition of the beta subunit of the Dictyostelium discoideum mitochondrial processing peptidase induces the expression of mitochondrial proteins.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
We cloned and characterized a cDNA encoding the Dictyostelium discoideum beta subunit of mitochondrial processing peptidase (Ddbeta-MPP). Western blot analysis of the mitochondrial subfractions revealed that Ddbeta-MPP is located in the mitochondrial matrix and membrane, whereas Dd(alpha)-MPP, another subunit of DdMPP, is located only in the matrix. Although expression of Ddbeta-MPP mRNA is down-regulated during early development, the level of the Ddbeta-MPP protein is constant throughout the Dictyostelium life cycle. In a transformant expressing the antisense RNA of the beta-MPP gene, unexpectedly, the beta-MPP protein increased about 1.8-fold relative to the wild type, and its mRNA increased 4.5-fold. Expression of other mitochondrial proteins, alpha-MPP and Cox IV, was also induced. These results suggest that antisense RNA inhibition of the beta-MPP gene induces gene expression of mitochondrial proteins, presumably in a retrograde signaling manner. This is the pathway of the transfer of information from the mitochondria to the nucleus.
|
| 巻・号 |
72(7)
|
| ページ |
1836-46
|
| 公開日 |
2008-7-1
|
| DOI |
10.1271/bbb.80106
|
| PII |
JST.JSTAGE/bbb/80106
|
| PMID |
18603769
|
| MeSH |
Animals
Dictyostelium / enzymology*
Gene Expression Regulation*
Metalloendopeptidases / antagonists & inhibitors*
Mitochondrial Proteins / genetics*
Protein Subunits
RNA, Antisense / physiology*
RNA, Messenger / analysis
|
| IF |
1.516
|
| 引用数 |
14
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 細胞性粘菌 |
G01338 |
