RRC ID 28733
Author Teo R, Lewis KJ, Forde JE, Ryves WJ, Reddy JV, Rogers BJ, Harwood AJ.
Title Glycogen synthase kinase-3 is required for efficient Dictyostelium chemotaxis.
Journal Mol Biol Cell
Abstract Glycogen synthase kinase-3 (GSK3) is a highly conserved protein kinase that is involved in several important cell signaling pathways and is associated with a range of medical conditions. Previous studies indicated a major role of the Dictyostelium homologue of GSK3 (gskA) in cell fate determination during morphogenesis of the fruiting body; however, transcriptomic and proteomic studies have suggested that GSK3 regulates gene expression much earlier during Dictyostelium development. To investigate a potential earlier role of GskA, we examined the effects of loss of gskA on cell aggregation. We find that cells lacking gskA exhibit poor chemotaxis toward cAMP and folate. Mutants fail to activate two important regulatory signaling pathways, mediated by phosphatidylinositol 3,4,5-trisphosphate (PIP(3)) and target of rapamycin complex 2 (TORC2), which in combination are required for chemotaxis and cAMP signaling. These results indicate that GskA is required during early stages of Dictyostelium development, in which it is necessary for both chemotaxis and cell signaling.
Volume 21(15)
Pages 2788-96
Published 2010-8-1
DOI 10.1091/mbc.E09-10-0891
PII E09-10-0891
PMID 20534815
PMC PMC2912363
MeSH Cell Aggregation / drug effects Cyclic AMP / biosynthesis Dictyostelium / cytology* Dictyostelium / drug effects Dictyostelium / enzymology* Dictyostelium / growth & development Folic Acid / pharmacology Glycogen Synthase Kinase 3 / metabolism* Models, Biological Mutation / genetics* Phosphatidylinositols / metabolism Phosphoric Monoester Hydrolases / metabolism Phosphorylation / drug effects Proto-Oncogene Proteins c-akt / metabolism Protozoan Proteins / metabolism Signal Transduction / drug effects
IF 3.905
Times Cited 17
Cellular slime molds G01747 G03261