論文 - 詳細
| RRC ID | 28740 |
|---|---|
| 著者 | Mohri K, Kiyota Y, Kuwayama H, Urushihara H. |
| タイトル | Temporal and non-permanent division of labor during sorocarp formation in the social amoeba Acytostelium subglobosum. |
| ジャーナル | Dev Biol |
| Abstract |
Somatic cell differentiation is crucial for the development of multicellular organisms. While the development of a fruiting body in Dictyostelium discoideum represents a simple model of this process with separation of stalk cells from the spore lineage, that of Acytostelium subglobosum is not accompanied by cell type separation. This species produces acellular stalks and, seemingly, all aggregated amoebae become spores; however, it possesses homologs for the stalk-cell marker genes of D. discoideum. In this study, we analyzed the spatio-temporal expression of A. subglobosum orthologs for D. discoideum stalk- or spore-lineage markers to clarify the developmental process of A. subglobosum. We first found that the prespore vesicles, which contained spore coat proteins, started to accumulate in the tip region and were observed in the entire sorogen throughout later development, confirming that all A. subglobosum cells became spores. The expression of a stalk-lineage gene ortholog, As-ecmA, started at the mound stage and was prominent in the protruding sorogen. Although two spore-lineage gene orthologs, As-cotD1 and -cotD2, were likewise detected shortly after cell aggregation and increased in intensity until tip formation, their expression diminished in the protruding sorogen. Double-fluorescence staining of these prestalk and prespore marker genes revealed that the expression of these marker genes was mutually exclusive and that expression switching occurred in the early tip. Our results indicate that A. subglobosum cells become committed to the spore lineage first, and then, while keeping this commitment intact, participate in stalk formation. Instead of the permanent division of labor observed in D. discoideum, A. subglobosum produces fruiting bodies by all cells contributing to the formation of the stalk as well as forming spores. |
| 巻・号 | 375(2) |
| ページ | 202-9 |
| 公開日 | 2013-3-15 |
| DOI | 10.1016/j.ydbio.2013.01.003 |
| PII | S0012-1606(13)00008-0 |
| PMID | 23313124 |
| MeSH | Amoeba / cytology Amoeba / genetics Amoeba / growth & development* Amoeba / ultrastructure Cell Lineage / genetics Cytoplasmic Vesicles / metabolism Cytoplasmic Vesicles / ultrastructure Dictyostelium / cytology Dictyostelium / genetics Gene Expression Regulation, Developmental Models, Biological Protozoan Proteins / genetics Protozoan Proteins / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism Spores, Protozoan / cytology Spores, Protozoan / genetics Spores, Protozoan / growth & development* Spores, Protozoan / ultrastructure Time Factors |
| IF | 2.896 |
| 引用数 | 8 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 細胞性粘菌 | G40383 G411082 G90140 G90141 G90142 |