論文 - 詳細
| RRC ID | 28831 |
|---|---|
| 著者 | Outani H, Okada M, Yamashita A, Nakagawa K, Yoshikawa H, Tsumaki N. |
| タイトル | Direct induction of chondrogenic cells from human dermal fibroblast culture by defined factors. |
| ジャーナル | PLoS One |
| Abstract |
The repair of large cartilage defects with hyaline cartilage continues to be a challenging clinical issue. We recently reported that the forced expression of two reprogramming factors (c-Myc and Klf4) and one chondrogenic factor (SOX9) can induce chondrogenic cells from mouse dermal fibroblast culture without going through a pluripotent state. We here generated induced chondrogenic (iChon) cells from human dermal fibroblast (HDF) culture with the same factors. We developed a chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells. The human iChon cells expressed marker genes for chondrocytes but not fibroblasts, and were derived from non-chondrogenic COL11A2-negative cells. The human iChon cells formed cartilage but not tumors in nude mice. This approach could lead to the preparation of cartilage directly from skin in human, without going through pluripotent stem cells. |
| 巻・号 | 8(10) |
| ページ | e77365 |
| 公開日 | 2013-8-16 |
| DOI | 10.1371/journal.pone.0077365 |
| PII | PONE-D-13-07592 |
| PMID | 24146984 |
| PMC | PMC3797820 |
| MeSH | Animals Bone Marrow Cells / cytology Bone Marrow Cells / metabolism Cartilage / cytology Cartilage / metabolism Cell Culture Techniques Cell Transdifferentiation / genetics* Chondrocytes / cytology* Chondrocytes / metabolism* Chondrogenesis / genetics Collagen Type XI / genetics Dermis / cytology* Enhancer Elements, Genetic Female Fibroblasts / cytology* Fibroblasts / metabolism* Gene Expression Profiling Genetic Vectors / genetics Humans Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / metabolism Kruppel-Like Factor 4 Kruppel-Like Transcription Factors / genetics Kruppel-Like Transcription Factors / metabolism Lentivirus / genetics Mice Proto-Oncogene Proteins c-myc / genetics Proto-Oncogene Proteins c-myc / metabolism Rats SOX9 Transcription Factor / genetics SOX9 Transcription Factor / metabolism Stem Cells / cytology Stem Cells / metabolism Transduction, Genetic |
| IF | 2.74 |
| 引用数 | 53 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| ラット | F344-Il2rgtm2kyo(strainID=979) |