| RRC ID |
28933
|
| Author |
Morrison A, Harwood A.
|
| Title |
A simple method of generating axenic derivatives of Dictyostelium strains.
|
| Journal |
Exp Cell Res
|
| Abstract |
We describe a generally applicable method of adapting Dictyostelium from growth on a bacterial food source to axenic growth. Cells are initially selected by growth on a plastic substratum but subsequently acquire the ability to grow in suspension culture. These strains can be transformed efficiently by DNA-mediated gene transfer.
|
| Volume |
199(2)
|
| Pages |
383-6
|
| Published |
1992-4-1
|
| DOI |
10.1016/0014-4827(92)90449-i
|
| PMID |
1544378
|
| MeSH |
Animals
Dictyostelium / growth & development*
Germ-Free Life*
Phenotype
Plasmids
|
| IF |
3.383
|
| Times Cited |
12
|
|
WOS Category
|
CELL BIOLOGY
ONCOLOGY
|
| Resource |
| Cellular slime molds |
S00470 |
