RRC ID |
28939
|
著者 |
Harloff C, Gerisch G, Noegel AA.
|
タイトル |
Selective elimination of the contact site A protein of Dictyostelium discoideum by gene disruption.
|
ジャーナル |
Genes Dev
|
Abstract |
The contact site A glycoprotein is a developmentally regulated cell-surface component expressed during the aggregation stage of Dictyostelium discoideum. This protein has been implicated in the EDTA-stable (Ca2(+)-independent) type of cell adhesion of aggregating cells. The gene coding for the contact site A protein was disrupted by homologous recombination, using a transformation vector that contained a 1.0-kb cDNA fragment as an insert. Transformants that did not express the protein were identified by colony immunoblotting. These transformants produced three truncated contact site A transcripts. One of them was controlled by the original contact site A promoter, as indicated by its strict developmental regulation and cAMP inducibility; the other two transcripts were transcribed from the actin 6 promoter of the vector. When cell adhesion was assayed in the transformants by agitating suspended cells in an agglutinometer, EDTA-stable adhesion was drastically reduced as compared to wild type, confirming that the contact site A glycoprotein acts as a cell-adhesion molecule. However, aggregation of the transformed cells on an agar surface was not remarkably altered. These results suggest that the contact site A glycoprotein is responsible for a 'fast' type of cell adhesion that is essential when aggregating cells are subjected to shear. When cells are not mechanically disturbed, a 'slow' type of adhesion mediated by other molecules is sufficient for their aggregation.
|
巻・号 |
3(12A)
|
ページ |
2011-9
|
公開日 |
1989-12-1
|
DOI |
10.1101/gad.3.12a.2011
|
PMID |
2515990
|
MeSH |
Blotting, Northern
Blotting, Southern
Cell Adhesion
Cell Adhesion Molecules / metabolism*
Dictyostelium / genetics*
Dictyostelium / physiology
Edetic Acid / pharmacology
Fungal Proteins / genetics
Fungal Proteins / metabolism*
Genes, Fungal*
Glycoproteins / genetics
Glycoproteins / metabolism*
Immunoblotting
Protozoan Proteins*
Restriction Mapping
Transcription, Genetic
Transformation, Genetic
|
IF |
9.527
|
引用数 |
77
|
WOS 分野
|
GENETICS & HEREDITY
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
リソース情報 |
細胞性粘菌 |
S00254 |