論文 - 詳細
| RRC ID | 28942 |
|---|---|
| 著者 | Knecht DA, Cohen SM, Loomis WF, Lodish HF. |
| タイトル | Developmental regulation of Dictyostelium discoideum actin gene fusions carried on low-copy and high-copy transformation vectors. |
| ジャーナル | Mol Cell Biol |
| Abstract |
The Dictyostelium discoideum genome contains an estimated 17 to 20 actin genes. We report the identification of a new member of this multigene family, actin 15, and its complete nucleotide sequence and transcription initiation sites. We constructed transformation vectors carrying either the actin 15 promoter fused to the neomycin phosphotransferase gene from transposon Tn903 or the actin 6 promoter fused to the neomycin phosphotransferase gene from Tn5. Cells transformed with the actin 15 vector carried less than five copies of vector DNA, while cells transformed with the actin 6 vector carried more than 200 copies. In both cases, the vector appeared to be integrated into the chromosome as a tandem array. Gene fusion RNAs transcribed from the actin 15 and actin 6 vectors were regulated like endogenous actin genes during D. discoideum development. DNA sequences required for temporal and cell type-specific regulation of these genes were contained within 2.8 kilobases of 5' noncoding DNA for actin 15 and 0.7 kilobases of 5' noncoding DNA for actin 6. |
| 巻・号 | 6(11) |
| ページ | 3973-83 |
| 公開日 | 1986-11-1 |
| DOI | 10.1128/mcb.6.11.3973-3983.1986 |
| PMID | 3025622 |
| PMC | PMC367161 |
| MeSH | Actins / genetics* Amino Acid Sequence Base Sequence Cloning, Molecular DNA Restriction Enzymes Dictyostelium / genetics* Dictyostelium / growth & development Genes* Genes, Fungal* Genetic Vectors* Nucleic Acid Hybridization |
| IF | 3.611 |
| 引用数 | 205 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 細胞性粘菌 | S00148 S00362 |