論文 - 詳細
| RRC ID | 28956 |
|---|---|
| 著者 | Ross FM, Newell PC. |
| タイトル | Streamers: chemotactic mutants of Dictyostelium discoideum with altered cyclic GMP metabolism. |
| ジャーナル | J Gen Microbiol |
| Abstract |
Mutants of Dictyostelium discoideum that developed huge aggregation streams in expanding clones were investigated using optical and biochemical techniques. Representatives of the six complementation groups previously identified (stmA-stmF) were found to be similar to the parental wild-type strain XP55 in both the extent and timing of their ability to initiate and relay chemotactic signals and in the formation of cyclic AMP receptors and phosphodiesterases. The mutants differed from the wild-type in producing an abnormal chemotactic (movement) response visible using both dark-field optics with synchronously aggregating amoebae on solid substrata and light scattering techniques with oxygenated cell suspensions. Mutants of complementation group stmF showed chemotactic movement responses lasting up to 520 s, rather than 100 s as seen in the parental and other strains. Measurements of cyclic GMP formed intracellularly in response to chemotactic pulses of cyclic AMP in stmF mutants showed that abnormally high concentrations of this nucleotide were formed within 10 s and were not rapidly degraded. A causal correlation between defective cyclic GMP metabolism and the altered chemotactic response is suggested, and a model is proposed that accounts for the formation of huge aggregation streams in clones of these mutants.U |
| 巻・号 | 127(2) |
| ページ | 339-50 |
| 公開日 | 1981-12-1 |
| DOI | 10.1099/00221287-127-2-339 |
| PMID | 6283012 |
| MeSH | Chemotaxis* Cyclic AMP / metabolism Cyclic GMP / metabolism* Dictyostelium / metabolism* Mutation Phosphoric Diester Hydrolases / metabolism |
| 引用数 | 159 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 細胞性粘菌 | S00209 |