論文 - 詳細
| RRC ID | 28978 |
|---|---|
| 著者 | de Hostos EL, Rehfuess C, Bradtke B, Waddell DR, Albrecht R, Murphy J, Gerisch G. |
| タイトル | Dictyostelium mutants lacking the cytoskeletal protein coronin are defective in cytokinesis and cell motility. |
| ジャーナル | J Cell Biol |
| Abstract |
Coronin is an actin-binding protein in Dictyostelium discoideum that is enriched at the leading edge of the cells and in projections of the cell surface called crowns. The polypeptide sequence of coronin is distinguished by its similarities to the beta-subunits of trimeric G proteins (E. L. de Hostos, B. Bradtke, F. Lottspeich, R. Guggenheim, and G. Gerisch, 1991. EMBO (Eur. Mol. Biol. Organ.) J. 10:4097-4104). To elucidate the in vivo function of coronin, null mutants have been generated by gene replacement. The mutant cells lacking coronin grow and migrate more slowly than wild-type cells. When these cor- cells grow in liquid medium they become multinucleate, indicating a role of coronin in cytokinesis. To explore this role, coronin has been localized in mitotic wild-type cells by immunofluorescence labeling. During separation of the daughter cells, coronin is strongly accumulated at their distal portions including the leading edges. This contrasts with the localization of myosin II in the cleavage furrow and suggests that coronin functions independently of the conventional myosin in facilitating cytokinesis. |
| 巻・号 | 120(1) |
| ページ | 163-73 |
| 公開日 | 1993-1-1 |
| DOI | 10.1083/jcb.120.1.163 |
| PMID | 8380174 |
| PMC | PMC2119478 |
| MeSH | Actins / metabolism Animals Base Sequence Cell Compartmentation Cell Division* Cell Movement* Chemotaxis Cyclic AMP / physiology Dictyostelium / cytology* Dictyostelium / genetics Fungal Proteins / metabolism* Gene Expression Microfilament Proteins / physiology* Molecular Sequence Data Mutagenesis, Insertional Mutation Myosins / metabolism Oligodeoxyribonucleotides / chemistry RNA, Fungal / genetics RNA, Messenger / genetics |
| IF | 8.811 |
| 引用数 | 200 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 細胞性粘菌 | S00417 S00550 |