| RRC ID |
28992
|
| 著者 |
Flick KM, Shaulsky G, Loomis WF.
|
| タイトル |
The wacA gene of Dictyostelium discoideum is a developmentally regulated member of the MIP family.
|
| ジャーナル |
Gene
|
| Abstract |
We isolated a cDNA from Dictyostelium discoideum that encodes a 30 kDa protein with significant similarity to members of the major intrinsic protein (MIP) family of membrane transporters. The most closely related protein in the public data bases is an aquaporin from Cicadella viridis which shows 34% identity. The cDNA was used to isolate and characterize genomic fragments carrying the Dictyostelium gene which we named wacA. Genomic probes were used to recognize wacA mRNA isolated at various stages of development. The results showed that the gene is developmentally regulated such that the mRNA first appears at 12 h of development and is retained throughout the remainder of development. In situ hybridization of whole mounts prepared at 15 h of development showed that wacA mRNA accumulates exclusively in prespore cells and is absent from prestalk cells. Although wacA expression is prespore specific, disruption of the gene by homologous recombination did not result in observable alterations in the formation of spores or their resistance to osmotic challenges.
|
| 巻・号 |
195(2)
|
| ページ |
127-30
|
| 公開日 |
1997-8-22
|
| DOI |
10.1016/s0378-1119(97)00078-4
|
| PII |
S0378-1119(97)00078-4
|
| PMID |
9305756
|
| MeSH |
Amino Acid Sequence
Animals
Aquaporins*
Chromosome Mapping
Cloning, Molecular
DNA Probes / genetics
Dictyostelium / genetics*
Dictyostelium / growth & development
Eye Proteins / genetics
Gene Expression Regulation, Developmental
Gene Expression Regulation, Fungal
Genes, Fungal
Genes, Protozoan
In Situ Hybridization
Insecta / genetics
Membrane Glycoproteins*
Molecular Sequence Data
Osmotic Pressure
Porins / genetics*
Protozoan Proteins*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Recombination, Genetic
Sequence Homology, Amino Acid
Spores, Fungal / genetics
Spores, Fungal / growth & development
Spores, Fungal / metabolism
|
| IF |
2.984
|
| 引用数 |
21
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| 細胞性粘菌 |
S00256
S90374 |
