Reference - Detail
| RRC ID | 29000 |
|---|---|
| Author | Chang WT, Thomason PA, Gross JD, Neweil PC. |
| Title | Evidence that the RdeA protein is a component of a multistep phosphorelay modulating rate of development in Dictyostelium. |
| Journal | EMBO J |
| Abstract |
We have isolated an insertional mutant of Dictyostelium discoideum that aggregated rapidly and formed spores and stalk cells within 14 h of development instead of the normal 24 h. We have shown by parasexual genetics that the insertion is in the rdeA locus and have cloned the gene. It encodes a predicted 28 kDa protein (RdeA) that is enriched in charged residues and is very hydrophilic. Constructs with the DNA for the c-Myc epitope or for the green fluorescent protein indicate that RdeA is not compartmentalized. RdeA displays homology around a histidine residue at amino acid 65 with members of the H2 module family of phosphotransferases that participate in multistep phosphoryl relays. Replacement of this histidine rendered the protein inactive. The mutant is complemented by transformation with the Ypd1 gene of Saccharomyces cerevisiae, itself an H2 module protein. We propose that RdeA is part of a multistep phosphorelay system that modulates the rate of development. |
| Volume | 17(10) |
| Pages | 2809-16 |
| Published | 1998-5-15 |
| DOI | 10.1093/emboj/17.10.2809 |
| PMID | 9582274 |
| PMC | PMC1170621 |
| MeSH | Adenylyl Cyclases / genetics Amino Acid Sequence Animals Base Sequence DNA, Complementary DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism Dictyostelium / growth & development* Dictyostelium / metabolism Fungal Proteins / genetics* Fungal Proteins / metabolism Gene Expression Intracellular Signaling Peptides and Proteins Molecular Sequence Data Mutagenesis Protein Kinases Protozoan Proteins* Saccharomyces cerevisiae Proteins* Sequence Homology, Amino Acid |
| IF | 9.889 |
| Times Cited | 64 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| Cellular slime molds | S00482 S00489 |