RRC ID 29003
著者 Bear JE, Rawls JF, Saxe CL 3rd.
タイトル SCAR, a WASP-related protein, isolated as a suppressor of receptor defects in late Dictyostelium development.
ジャーナル J Cell Biol
Abstract G protein-coupled receptors trigger the reorganization of the actin cytoskeleton in many cell types, but the steps in this signal transduction cascade are poorly understood. During Dictyostelium development, extracellular cAMP functions as a chemoattractant and morphogenetic signal that is transduced via a family of G protein-coupled receptors, the cARs. In a strain where the cAR2 receptor gene is disrupted by homologous recombination, the developmental program arrests before tip formation. In a genetic screen for suppressors of this phenotype, a gene encoding a protein related to the Wiskott-Aldrich Syndrome protein was discovered. Loss of this protein, which we call SCAR (suppressor of cAR), restores tip formation and most later development to cAR2(-) strains, and causes a multiple-tip phenotype in a cAR2(+) strain as well as leading to the production of extremely small cells in suspension culture. SCAR-cells have reduced levels of F-actin staining during vegetative growth, and abnormal cell morphology and actin distribution during chemotaxis. Uncharacterized homologues of SCAR have also been identified in humans, mouse, Caenorhabditis elegans, and Drosophila. These data suggest that SCAR may be a conserved negative regulator of G protein-coupled signaling, and that it plays an important role in regulating the actin cytoskeleton.
巻・号 142(5)
ページ 1325-35
公開日 1998-9-7
DOI 10.1083/jcb.142.5.1325
PMID 9732292
PMC PMC2149354
MeSH Amino Acid Sequence Animals Cell Movement / genetics Cell Size / genetics Cloning, Molecular Dictyostelium / growth & development* Fungal Proteins / chemistry GTP-Binding Proteins / physiology* Gene Targeting Immunohistochemistry Molecular Sequence Data Phenotype Proteins / chemistry* Protozoan Proteins* RNA, Messenger / metabolism Receptors, Cell Surface / metabolism Sequence Alignment Sequence Analysis, DNA Signal Transduction / physiology Suppression, Genetic / genetics Wiskott-Aldrich Syndrome / genetics Wiskott-Aldrich Syndrome Protein
IF 8.811
引用数 208
WOS 分野 CELL BIOLOGY
リソース情報
細胞性粘菌 S90307