論文 - 詳細
| RRC ID | 29014 |
|---|---|
| 著者 | Gabriel D, Hacker U, Köhler J, Müller-Taubenberger A, Schwartz JM, Westphal M, Gerisch G. |
| タイトル | The contractile vacuole network of Dictyostelium as a distinct organelle: its dynamics visualized by a GFP marker protein. |
| ジャーナル | J Cell Sci |
| Abstract |
The contractile vacuole system is an osmoregulatory organelle composed of cisternae and interconnecting ducts. Large cisternae act as bladders that periodically fuse with the plasma membrane, forming pores to expel water. To visualize the entire network in vivo and to identify constituents of the vacuolar complex in cell fractions, we introduced a specific marker into Dictyostelium cells, GFP-tagged dajumin. The C-terminal, GFP-tagged region of this transmembrane protein is responsible for sorting to the contractile vacuole complex. Dajumin-GFP negligibly associates with the plasma membrane, indicating its retention during discharge of the bladder. Fluorescent labeled cell-surface constituents are efficiently internalized by endocytosis, while no significant cycling through the contractile vacuole is observed. Endosomes loaded with yeast particles or a fluid-phase marker indicate sharp separation of the endocytic pathway from the contractile vacuole compartment. Even after dispersion of the contractile vacuole system during mitosis, dajumin-GFP distinguishes the vesicles from endosomes, and visualizes post-mitotic re-organization of the network around the nucleus. Highly discriminative sorting and membrane fusion mechanisms are proposed to account for the sharp separation of the contractile vacuole and endosomal compartments. Evidence for a similar compartment in other eukaryotic cells is discussed. |
| 巻・号 | 112 ( Pt 22) |
| ページ | 3995-4005 |
| 公開日 | 1999-11-1 |
| DOI | 10.1242/jcs.112.22.3995 |
| PMID | 10547360 |
| MeSH | Animals Biological Transport Cell Adhesion Molecules / genetics Cell Compartmentation Cell Membrane / metabolism Dictyostelium / cytology Dictyostelium / genetics Dictyostelium / metabolism* Endocytosis Endosomes / metabolism Fluorescent Antibody Technique Green Fluorescent Proteins Intracellular Membranes / metabolism Luminescent Proteins / genetics Membrane Fusion Membrane Glycoproteins / genetics Microscopy, Confocal Mitosis / physiology Organelles / metabolism* Protozoan Proteins* Recombinant Fusion Proteins / genetics Time Factors Vacuoles / metabolism* Vacuoles / physiology |
| IF | 4.573 |
| 引用数 | 62 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 細胞性粘菌 | S00455 |