RRC ID 29031
Author Roelofs J, Meima M, Schaap P, Van Haastert PJ.
Title The Dictyostelium homologue of mammalian soluble adenylyl cyclase encodes a guanylyl cyclase.
Journal EMBO J
Abstract A new Dictyostelium discoideum cyclase gene was identified that encodes a protein (sGC) with 35% similarity to mammalian soluble adenylyl cyclase (sAC). Gene disruption of sGC has no effect on adenylyl cyclase activity and results in a >10-fold reduction in guanylyl cyclase activity. The scg- null mutants show reduced chemotactic sensitivity and aggregate poorly under stringent conditions. With Mn(2+)/GTP as substrate, most of the sGC activity is soluble, but with the more physiological Mg(2+)/GTP the activity is detected in membranes and stimulated by GTPgammaS. Unexpectedly, orthologues of sGC and sAC are present in bacteria and vertebrates, but absent from Drosophila melanogaster, Caenorhabditis elegans, Arabidopsis thaliana and Saccharomyces cerevisiae.
Volume 20(16)
Pages 4341-8
Published 2001-8-15
DOI 10.1093/emboj/20.16.4341
PMID 11500361
PMC PMC125260
MeSH Adenylyl Cyclases / classification Adenylyl Cyclases / genetics* Adenylyl Cyclases / metabolism Amino Acid Sequence Animals Catalytic Domain Dictyostelium / enzymology* Dictyostelium / genetics Guanylate Cyclase / classification Guanylate Cyclase / genetics* Guanylate Cyclase / metabolism Humans Mammals Molecular Sequence Data Phenotype Phylogeny Sequence Homology, Amino Acid Solubility
IF 9.889
Times Cited 54
Cellular slime molds S00337