RRC ID 29047
Author Gerisch G, Heuser J, Clarke M.
Title Tubular-vesicular transformation in the contractile vacuole system of Dictyostelium.
Journal Cell Biol Int
Abstract The contractile vacuole complex of Dictyostelium is the paradigm of a membrane system that undergoes tubular-vesicular transitions during its regular cycle of activities. This system acts as an osmoregulatory organelle in freshwater amoebae and protozoa. It collects fluid in a network of tubules and cisternae, and pumps it out of the cell through transient pores in the plasma membrane. Tubules and vacuoles are interconvertible. The tubular channels are associated with the cortical actin network and are capable of moving and fusing. The contractile vacuole complex is separate from vesicles of the endosomal pathway and preserves its identity in a dispersed state during cell division. We outline techniques to visualize the contractile vacuole system by electron and light microscopy. Emphasis is placed on GFP-fusion proteins that allow visualization of the dynamics of the contractile vacuole network in living cells. Proteins that control activities of this specialized organelle in Dictyostelium have been conserved during evolution and also regulate membrane trafficking in man.
Volume 26(10)
Pages 845-52
Published 2002-1-1
DOI 10.1006/cbir.2002.0938
PII S1065699502909388
PMID 12421575
MeSH Animals Contractile Proteins / metabolism Dictyostelium / metabolism Dictyostelium / ultrastructure* Intracellular Membranes / metabolism Intracellular Membranes / ultrastructure Microtubules / metabolism Microtubules / ultrastructure* Protein Transport / physiology Transport Vesicles / metabolism Transport Vesicles / ultrastructure* Vacuoles / metabolism Vacuoles / ultrastructure* Water-Electrolyte Balance / physiology
IF 2.127
Times Cited 45
Cellular slime molds S00476