論文 - 詳細
| RRC ID | 29048 |
|---|---|
| 著者 | Bosgraaf L, Russcher H, Snippe H, Bader S, Wind J, Van Haastert PJ. |
| タイトル | Identification and characterization of two unusual cGMP-stimulated phoshodiesterases in dictyostelium. |
| ジャーナル | Mol Biol Cell |
| Abstract |
Recently, we recognized two genes, gbpA and gbpB, encoding putative cGMP-binding proteins with a Zn(2+)-hydrolase domain and two cyclic nucleotide binding domains. The Zn(2+)-hydrolase domains belong to the superfamily of beta-lactamases, also harboring a small family of class II phosphodiesterases from bacteria and lower eukaryotes. Gene inactivation and overexpression studies demonstrate that gbpA encodes the cGMP-stimulated cGMP-phosphodiesterase that was characterized biochemically previously and was shown to be involved in chemotaxis. cAMP neither activates nor is a substrate of GbpA. The gbpB gene is expressed mainly in the multicellular stage and seems to encode a dual specificity phosphodiesterase with preference for cAMP. The enzyme hydrolyses cAMP approximately 9-fold faster than cGMP and is activated by cAMP and cGMP with a K(A) value of approximately 0.7 and 2.3 microM, respectively. Cells with a deletion of the gbpB gene have increased basal and receptor stimulated cAMP levels and are sporogeneous. We propose that GbpA and GbpB hydrolyze the substrate in the Zn(2+)-hydrolase domain, whereas the cyclic nucleotide binding domains mediate activation. The human cGMP-stimulated cAMP/cGMP phosphodiesterase has similar biochemical properties, but a completely different topology: hydrolysis takes place by a class I catalytic domain and GAF domains mediate cGMP activation. |
| 巻・号 | 13(11) |
| ページ | 3878-89 |
| 公開日 | 2002-11-1 |
| DOI | 10.1091/mbc.e02-05-0302 |
| PMID | 12429832 |
| PMC | PMC133600 |
| MeSH | 3',5'-Cyclic-GMP Phosphodiesterases / chemistry 3',5'-Cyclic-GMP Phosphodiesterases / genetics 3',5'-Cyclic-GMP Phosphodiesterases / metabolism* Amino Acid Sequence Animals Cyclic AMP / metabolism Cyclic GMP / metabolism Dictyostelium / cytology Dictyostelium / enzymology* Dictyostelium / genetics Dictyostelium / physiology Gene Targeting Humans Molecular Sequence Data Phenotype Protein Structure, Tertiary Sequence Alignment |
| IF | 3.791 |
| 引用数 | 35 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 細胞性粘菌 | S00142 S00339 S00340 |