| RRC ID |
29061
|
| Author |
Tsujioka M, Yamamoto T, Thompson CR, Kay RR, Maeda M.
|
| Title |
Novel development rescuing factors (DRFs) secreted by the developing Dictyostelium cells, that are involved in the restoration of a mutant lacking MAP-kinase ERK2.
|
| Journal |
Zoolog Sci
|
| Abstract |
We found novel development rescuing factors (DRFs) secreted from developing Dictyostelium cells, by using a mutant (erkB-) which is missing MAP-kinase ERK2 as a test strain for bioassay. The mutant erkB- fails to undergo multicellular morphogenesis due to impaired cAMP signaling. However, such developmental defect can be restored by the presence of low-molecular weight DRFs that are secreted from developing wild-type cells. We previously showed that DIF-1 (Differentiation-Inducing Factor 1 for stalk cells) possesses this activity, indicating a newly discovered role of DIF-1. Surprisingly, however, the mutant dmtA-, which is incapable of DIF-1 synthesis still exerts a strong inducing activity of the multicellular morphogenesis of erkB-. After analysis of HPLC fractions of conditioned media prepared from both wild type Ax2 and dmtA- strains revealed that both strains secrete at least two novel DRF activities with DIF-like mobility. However, these activities were not derived from other DIFs such as DIF-2 and DIF-3. Identification of these DRFs found in this study would provide insight into the mechanism by which the development of the erkB- mutant is restored and how these factors act in the normal development of Dictyostelium.
|
| Volume |
21(8)
|
| Pages |
829-34
|
| Published |
2004-8-1
|
| DOI |
10.2108/zsj.21.829
|
| PII |
JST.JSTAGE/zsj/21.829
|
| PMID |
15333995
|
| MeSH |
Animals
Cell Differentiation / physiology*
Chromatography, High Pressure Liquid
Dictyostelium / metabolism*
Gene Expression Regulation, Developmental*
Hexanones / metabolism*
Mitogen-Activated Protein Kinase 1 / metabolism*
Morphogenesis
Pentanones / metabolism
Signal Transduction / physiology*
|
| IF |
0.843
|
| Times Cited |
3
|
|
WOS Category
|
ZOOLOGY
|
| Resource |
| Cellular slime molds |
S00409 |
