論文 - 詳細
| RRC ID | 29063 |
|---|---|
| 著者 | Kosta A, Roisin-Bouffay C, Luciani MF, Otto GP, Kessin RH, Golstein P. |
| タイトル | Autophagy gene disruption reveals a non-vacuolar cell death pathway in Dictyostelium. |
| ジャーナル | J Biol Chem |
| Abstract |
Types of cell death include apoptosis, necrosis, and autophagic cell death. The latter can be defined as death of cells containing autophagosomes, autophagic bodies, and/or vacuoles. Are autophagy and vacuolization causes, consequences, or side effects in cell death with autophagy? Would control of autophagy suffice to control this type of cell death? We disrupted the atg1 autophagy gene in Dictyostelium discoideum, a genetically tractable model for developmental autophagic vacuolar cell death. The procedure that induced autophagy, vacuolization, and death in wild-type cells led in atg1 mutant cells to impaired autophagy and to no vacuolization, demonstrating that atg1 is required for vacuolization. Unexpectedly, however, cell death still took place, with a non-vacuolar and centrally condensed morphology. Thus, a cell death mechanism that does not require vacuolization can operate in this cell death model showing conspicuous vacuolization. The revelation of non-vacuolar cell death in this protist by autophagy gene disruption is reminiscent of caspase inhibition revealing necrotic cell death in animal cells. Thus, hidden alternative cell death pathways may be found across kingdoms and for diverse types of cell death. |
| 巻・号 | 279(46) |
| ページ | 48404-9 |
| 公開日 | 2004-11-12 |
| DOI | 10.1074/jbc.M408924200 |
| PII | S0021-9258(20)67983-2 |
| PMID | 15358773 |
| MeSH | Animals Autophagy / genetics* Cell Death / physiology Dictyostelium* / genetics Dictyostelium* / metabolism Dictyostelium* / ultrastructure Gene Silencing Genetic Complementation Test Protozoan Proteins / genetics* Protozoan Proteins / metabolism Vacuoles / metabolism Vacuoles / ultrastructure |
| IF | 4.238 |
| 引用数 | 77 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 細胞性粘菌 | S00446 |