論文 - 詳細
| RRC ID | 29073 |
|---|---|
| 著者 | Zhukovskaya NV, Fukuzawa M, Yamada Y, Araki T, Williams JG. |
| タイトル | The Dictyostelium bZIP transcription factor DimB regulates prestalk-specific gene expression. |
| ジャーナル | Development |
| Abstract |
The ecmA gene is specifically expressed in prestalk cells and its transcription is induced by the chlorinated hexaphenone DIF-1. We have purified a novel bZIP transcription factor, DimB, by affinity chromatography on two spatially separated ecmA promoter fragments. Mutagenesis of the cap-site proximal DimB-binding site (the -510 site) greatly decreases ecmA expression in the pstO cells, which comprise the rear half of the prestalk zone, and also in the Anterior-Like Cells, which lie scattered throughout the prespore region. However, DimB is not essential for normal expression of the ecmA gene, instead it spatially limits its expression; ecmA is relatively highly expressed in the subset of prestalk cells that coats the prestalk zone, but in slugs of a DimB-null strain, ecmA is highly expressed throughout the prestalk zone. Because the -510 site is required for correct ecmA expression, we posit a separate activator protein that competes with DimB for binding to the -510 site. DimB rapidly accumulates in the nucleus when cells are exposed to DIF-1, and ChIP analysis shows that, in the presence of extracellular cAMP, DIF-1 causes DimB to associate with the ecmA promoter in vivo. Thus, DIF-1 regulates DimB activity to generate a gradient of ecmA expression in the prestalk zone of the slug. |
| 巻・号 | 133(3) |
| ページ | 439-48 |
| 公開日 | 2006-2-1 |
| DOI | 10.1242/dev.02190 |
| PII | dev.02190 |
| PMID | 16396914 |
| MeSH | Amino Acid Sequence Animals Basic-Leucine Zipper Transcription Factors / genetics Basic-Leucine Zipper Transcription Factors / metabolism* Binding Sites Cell Differentiation Cell Nucleus / metabolism Cyclic AMP / metabolism Dictyostelium / cytology Dictyostelium / genetics Dictyostelium / metabolism* Extracellular Matrix Proteins / genetics Extracellular Matrix Proteins / metabolism* Gene Expression Regulation* Hexanones / metabolism Molecular Sequence Data Morphogenesis / physiology Point Mutation Promoter Regions, Genetic Protozoan Proteins / genetics Protozoan Proteins / metabolism* Sequence Alignment |
| IF | 5.611 |
| 引用数 | 48 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 細胞性粘菌 | S90379 |