論文 - 詳細
| RRC ID | 29076 |
|---|---|
| 著者 | Bader S, Kortholt A, Snippe H, Van Haastert PJ. |
| タイトル | DdPDE4, a novel cAMP-specific phosphodiesterase at the surface of dictyostelium cells. |
| ジャーナル | J Biol Chem |
| Abstract |
Dictyostelium discoideum cells possess multiple cyclic nucleotide phosphodiesterases that belong either to class I enzymes that are present in all eukaryotes or to the rare beta-lactamase class II. We describe here the identification and characterization of DdPDE4, the third class I enzyme of Dictyostelium. The deduced amino acid sequence predicts that DdPDE4 has a leader sequence, two transmembrane segments, and an extracellular catalytic domain that exhibits a high degree of homology with human cAMP-specific PDE8. Expression of the catalytic domain of DdPDE4 shows that the enzyme is a cAMP-specific phosphodiesterase with a K(m) of 10 microm; cGMP is hydrolyzed at least 100-fold more slowly. The full-length protein is shown to be membrane-bound with catalytic activity exposed to the extracellular medium. Northern blots and activity measurements reveal that expression of DdPDE4 is low during single cell stages and increases at 9 h of starvation, corresponding with mound stage. A function during multicellular development is confirmed by the phenotype of ddpde4(-) knock-out strains, showing normal aggregation but impaired development from the mound stage on. These results demonstrate that DdPDE4 is a unique membrane-bound phosphodiesterase with an extracellular catalytic domain regulating intercellular cAMP during multicellular development. |
| 巻・号 | 281(29) |
| ページ | 20018-26 |
| 公開日 | 2006-7-21 |
| DOI | 10.1074/jbc.M600040200 |
| PII | S0021-9258(19)76316-9 |
| PMID | 16644729 |
| MeSH | 3',5'-Cyclic-AMP Phosphodiesterases / chemistry 3',5'-Cyclic-AMP Phosphodiesterases / genetics* 3',5'-Cyclic-AMP Phosphodiesterases / metabolism* Amino Acid Sequence Animals Cell Membrane / enzymology* Conserved Sequence Cyclic AMP Cyclic Nucleotide Phosphodiesterases, Type 4 DNA Primers Dictyostelium / enzymology* Dictyostelium / genetics Gene Deletion Humans Molecular Sequence Data Phenotype Polymerase Chain Reaction Protozoan Proteins / metabolism Sequence Alignment Sequence Homology, Amino Acid |
| IF | 4.238 |
| 引用数 | 16 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 細胞性粘菌 | S00336 |