論文 - 詳細
RRC ID | 29091 |
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著者 | Riedl J, Crevenna AH, Kessenbrock K, Yu JH, Neukirchen D, Bista M, Bradke F, Jenne D, Holak TA, Werb Z, Sixt M, Wedlich-Soldner R. |
タイトル | Lifeact: a versatile marker to visualize F-actin. |
ジャーナル | Nat Methods |
Abstract |
Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells. |
巻・号 | 5(7) |
ページ | 605-7 |
公開日 | 2008-7-1 |
DOI | 10.1038/nmeth.1220 |
PII | nmeth.1220 |
PMID | 18536722 |
PMC | PMC2814344 |
MeSH | Actins / metabolism* Animals Cells, Cultured Coloring Agents / chemistry* Cytoskeleton / metabolism Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Humans Mice Microscopy, Fluorescence Peptides / chemistry* Rats Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Staining and Labeling / methods* |
IF | 30.822 |
引用数 | 1213 |
WOS 分野 | BIOCHEMICAL RESEARCH METHODS |
リソース情報 | |
細胞性粘菌 | S00444 G90096 |