RRC ID 29091
著者 Riedl J, Crevenna AH, Kessenbrock K, Yu JH, Neukirchen D, Bista M, Bradke F, Jenne D, Holak TA, Werb Z, Sixt M, Wedlich-Soldner R.
タイトル Lifeact: a versatile marker to visualize F-actin.
ジャーナル Nat Methods
Abstract Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells.
巻・号 5(7)
ページ 605-7
公開日 2008-7-1
DOI 10.1038/nmeth.1220
PII nmeth.1220
PMID 18536722
PMC PMC2814344
MeSH Actins / metabolism* Animals Cells, Cultured Coloring Agents / chemistry* Cytoskeleton / metabolism Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Humans Mice Microscopy, Fluorescence Peptides / chemistry* Rats Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Staining and Labeling / methods*
IF 30.822
引用数 1213
WOS 分野 BIOCHEMICAL RESEARCH METHODS
リソース情報
細胞性粘菌 S00444 G90096