RRC ID 29093
Author Nagasaki A, Kanada M, Uyeda TQ.
Title Cell adhesion molecules regulate contractile ring-independent cytokinesis in Dictyostelium discoideum.
Journal Cell Res
Abstract To investigate the roles of substrate adhesion in cytokinesis, we established cell lines lacking paxillin (PAXB) or vinculin (VINA), and those expressing the respective GFP fusion proteins in Dictyostelium discoideum. As in mammalian cells, GFP-PAXB and GFP-VINA formed focal adhesion-like complexes on the cell bottom. paxB(-) cells in suspension grew normally, but on substrates, often failed to divide after regression of the furrow. The efficient cytokinesis of paxB(-) cells in suspension is not because of shear forces to assist abscission, as they divided normally in static suspension culture as well. Double knockout strains lacking mhcA, which codes for myosin II, and paxB or vinA displayed more severe cytokinetic defects than each single knockout strain. In mitotic wild-type cells, GFP-PAXB was diffusely distributed on the basal membrane, but was strikingly condensed along the polar edges in mitotic mhcA(-) cells. These results are consistent with our idea that Dictyostelium displays two forms of cytokinesis, one that is contractile ring-dependent and adhesion-independent, and the other that is contractile ring-independent and adhesion-dependent, and that the latter requires PAXB and VINA. Furthermore, that paxB(-) cells fail to divide normally in the presence of substrate adhesion suggests that this adhesion molecule may play additional signaling roles.
Volume 19(2)
Pages 236-46
Published 2009-2-1
DOI 10.1038/cr.2008.318
PII cr2008318
PMID 19065153
MeSH Animals Cell Adhesion Molecules / genetics Cell Adhesion Molecules / metabolism* Contractile Proteins / genetics Contractile Proteins / metabolism* Cytokinesis / genetics Cytokinesis / physiology* Dictyostelium / genetics Dictyostelium / metabolism Dictyostelium / physiology* Gene Knockout Techniques Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Paxillin / genetics Paxillin / metabolism Phenotype Vinculin / genetics Vinculin / metabolism
IF 20.507
Times Cited 24
Cellular slime molds S00317 S00322 S90027 S00551