論文 - 詳細
| RRC ID | 29124 |
|---|---|
| 著者 | Li B, Kim H, Beers M, Kemphues K. |
| タイトル | Different domains of C. elegans PAR-3 are required at different times in development. |
| ジャーナル | Dev Biol |
| Abstract |
Polarity is a fundamental cellular feature that is critical for generating cell diversity and maintaining organ functions during development. In C. elegans, the one-cell embryo is polarized via asymmetric localization of the PAR proteins, which in turn are required to establish the future anterior-posterior axis of the embryo. PAR-3, a conserved PDZ domain-containing protein, acts with PAR-6 and PKC-3 (atypical protein kinase; aPKC) to regulate cell polarity and junction formation in a variety of cell types. To understand how PAR-3 localizes and functions during C. elegans development, we produced targeted mutations and deletions of conserved domains of PAR-3 and examined the localization and function of the GFP-tagged proteins in C. elegans embryos and larvae. We find that CR1, the PAR-3 self-oligomerization domain, is required for PAR-3 cortical distribution and function only during early embryogenesis and that PDZ2 is required for PAR-3 to accumulate stably at the cell periphery in early embryos and at the apical surface in pharyngeal and intestinal epithelial cells. We also show that phosphorylation at S863 by PKC-3 is not essential in early embryogenesis, but is important in later development. Surprisingly neither PDZ1 nor PDZ3 are essential for localization or function. Our results indicate that the different domains and phosphorylated forms of PAR-3 can have different roles during C. elegans development. |
| 巻・号 | 344(2) |
| ページ | 745-57 |
| 公開日 | 2010-8-15 |
| DOI | 10.1016/j.ydbio.2010.05.506 |
| PII | S0012-1606(10)00809-2 |
| PMID | 20678977 |
| PMC | PMC2915941 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans / physiology Cell Polarity / genetics Cell Polarity / physiology* Epithelial Cells / cytology Epithelial Cells / metabolism Epithelial Cells / physiology Membrane Proteins Neoplasm Proteins PDZ Domains Poly(ADP-ribose) Polymerases / genetics Poly(ADP-ribose) Polymerases / metabolism Protein Kinase C / genetics Protein Kinase C / metabolism* Protein Kinase C / physiology Proteins / genetics Proteins / metabolism Transcription Factors / genetics Transcription Factors / metabolism |
| IF | 2.896 |
| 引用数 | 26 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | par-3(tm2010) |