Reference - Detail
| RRC ID | 29130 |
|---|---|
| Author | Kanegae Y, Lee G, Sato Y, Tanaka M, Nakai M, Sakaki T, Sugano S, Saito I. |
| Title | Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. |
| Journal | Nucleic Acids Res |
| Abstract |
A recombinant adenovirus (Ad) expressing Cre recombinase derived from bacteriophage P1 was constructed. To assay the Cre activity in mammalian cells, another recombinant Ad bearing an on/off-switching reporter unit, where a LacZ-expression unit can be activated by the Cre-mediated excisional deletion of an interposed stuffer DNA, was also constructed. Co-infection experiments together with the Cre-expressing and the reporter recombinant Ads showed that the Cre-mediated switching of gene expression was detected in nearly 100% of cultured CV1, HeLa and Jurkat cells. These results suggest that the recombinant Ad efficiently expressed functional Cre and offers a basis for establishing a powerful on/off switching strategy of gene expression in cultured mammalian cells and presumably in transgenic animals. The method is also applicable to construction of recombinant Ad bearing a gene the expression of which is deleterious to propagation of recombinant Ad. |
| Volume | 23(19) |
| Pages | 3816-21 |
| Published | 1995-10-11 |
| DOI | 10.1093/nar/23.19.3816 |
| PII | 5w0150 |
| PMID | 7479022 |
| PMC | PMC307296 |
| MeSH | Adenoviridae / genetics* Animals Base Sequence Blotting, Southern Cell Line DNA Nucleotidyltransferases / genetics* Escherichia coli / genetics Gene Expression Regulation* Haplorhini HeLa Cells Humans Integrases* Kidney Molecular Sequence Data Promoter Regions, Genetic Recombinant Proteins / metabolism T-Lymphocytes Transcriptional Activation Transfection Viral Proteins* beta-Galactosidase / genetics beta-Galactosidase / metabolism |
| IF | 11.502 |
| Times Cited | 601 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 97 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| DNA material | pAxcw (RDB00917) pAxc (RDB00918) pCALNLw (RDB01674) pxCANCre (RDB01675) pxCANLacZ (RDB01676) pULwL (RDB01677) pAxCAwt (RDB01678) pCALwL (RDB01679) pCALNLZ (RDB01680) AxCALacZ (RDB01745) AxCANCre (RDB01748) AxCANLacZ (RDB01749) AxCALNLNZ (RDB01750) pCALNL5 (RDB01862) |