論文 - 詳細
| RRC ID | 29132 |
|---|---|
| 著者 | Kimura N, Fukushima A, Oda K, Nakada S. |
| タイトル | An in vivo study of the replication origin in the influenza virus complementary RNA. |
| ジャーナル | J Biochem |
| Abstract |
A new in vivo replication system for influenza virus was developed by using the clone 76 cell line, in which the viral RNA polymerase and nucleoprotein genes can be expressed in response to dexamethasone. The chimeric NS-chloramphenicol acetyltransferase (CAT) RNAs in the sense and antisense orientations positioned between the 5'- and 3'-terminal sequences of the influenza virus RNA segment 8 can be replicated [both genomic RNA (vRNA) and complementary RNA (cRNA) were transcribed] in the clone 76 cells treated with dexamethasone. These data indicate that three RNA polymerase proteins (PB1, PB2, and PA) and nucleoprotein are sufficient for replication of the influenza virus genome. Analysis of mutant cRNAs containing a base-substitution or a deletion in the 3'-conserved terminal 13 nucleotides revealed that important cis elements in the cRNA for vRNA synthesis reside at positions 2, 3, and 7 to 13 nucleotides from the 3'-end. |
| 巻・号 | 113(1) |
| ページ | 88-92 |
| 公開日 | 1993-1-1 |
| DOI | 10.1093/oxfordjournals.jbchem.a124009 |
| PMID | 7681056 |
| MeSH | Animals Base Sequence Blotting, Northern Cell Line Chloramphenicol O-Acetyltransferase / genetics Cloning, Molecular Dexamethasone / pharmacology Influenza A virus / genetics* Influenza A virus / physiology Mice Molecular Sequence Data Mutation Nucleic Acid Conformation Polymerase Chain Reaction RNA / biosynthesis RNA / genetics RNA, Antisense RNA, Complementary RNA, Viral / biosynthesis RNA, Viral / genetics* Recombinant Fusion Proteins / genetics Transcription, Genetic Viral Nonstructural Proteins / genetics Virus Replication / genetics* |
| IF | 2.476 |
| 引用数 | 10 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 74 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pT7/NS-CATc (RDB02055) pT7/NS-CATc-mut (1U-C) (RDB02056) pT7/NS-CATc-mut (2C-G) (RDB02057) pT7/NS-CATc-mut (3A-C) (RDB02058) pT7/NS-CATc-mut (5C-A) (RDB02059) pT7/NS-CATc-mut (6U-A) (RDB02060) pT7/NS-CATc-mut (7U-A) (RDB02061) pT7/NS-CATc-mut (8U-C) (RDB02062) pT7/NS-CATc-mut (9G-C) (RDB02063) pT7/NS-CATc-mut (10U-delta) (RDB02064) pT7/NS-CATc-mut (11U-A) (RDB02065) pT7/NS-CATc-mut (12C-G) (RDB02066) pT7/NS-CATc-mut (13C-G) (RDB02067) pT7/delta NSc (RDB02068) |