| RRC ID |
29135
|
| Author |
Nakamura Y, Oda K, Nakada S.
|
| Title |
Growth complementation of influenza virus temperature-sensitive mutants in mouse cells which express the RNA polymerase and nucleoprotein genes.
|
| Journal |
J Biochem
|
| Abstract |
In order to establish cell lines which complement the growth of temperature-sensitive (ts) mutants of influenza virus, three RNA polymerase and nucleoprotein (NP) genes each linked to the mouse mammary tumor virus LTR were cloned into the bovine papillomavirus vector DNA. After co-transfection of mouse C127 cells with these recombinant plasmids, a cell line, clone 76, in which the expression of the three polymerase and NP genes could be stimulated by dexamethasone, was established. The clone 76 cells could complement the growth of ts-mutants defective in one of the polymerase subunit genes at the nonpermissive temperature in response to dexamethasone. The results suggest that the simultaneous expression of the three polymerase genes in the same compartment of protein synthesis machinery is required for an efficient complementation of ts-mutant growth.
|
| Volume |
110(3)
|
| Pages |
395-401
|
| Published |
1991-9-1
|
| DOI |
10.1093/oxfordjournals.jbchem.a123592
|
| PMID |
1663110
|
| MeSH |
Animals
Bovine papillomavirus 1 / genetics
Cell Line
Cell Line, Transformed
DNA, Recombinant / metabolism
DNA-Directed RNA Polymerases / genetics*
DNA-Directed RNA Polymerases / metabolism
Dexamethasone / pharmacology
Gene Expression / drug effects
Genes, Viral / drug effects
Genetic Complementation Test
Genetic Vectors
Influenza A virus / genetics*
Influenza A virus / growth & development
Mammary Tumor Virus, Mouse / genetics
Mice
Mutation
Nucleoproteins / genetics*
Nucleoproteins / metabolism
Plasmids
Restriction Mapping
Temperature
Transfection
|
| IF |
2.476
|
| Times Cited |
18
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
pBMSA (RDB01968)
pBMSA-PB2 (RDB01969)
pBMSA-PB1 (RDB01970)
pBMSA-PA (RDB01971)
pBMSA-NP (RDB01972) |
