RRC ID 29139
Author Takemoto Y, Sato M, Furuta M, Hashimoto Y.
Title Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins.
Journal DNA Cell Biol
Abstract We have generated cDNA expression vectors that efficiently produce tagged proteins. The newly introduced cloning site of this plasmid facilitates subcloning of cDNA in the lambda gt11 phage into the plasmid vector. Because the cDNA is inserted next to the motifs of the tagged DNA sequence, the protein produced by the tag sequence-coupled cDNA is easily detected by Western blot analysis or immunoprecipitation using commercially available antibodies. The double-tagged protein significantly enhances the efficiency of Western blot and immunoprecipitation detection as compared with the single-tagged protein.
Volume 16(7)
Pages 893-6
Published 1997-7-1
DOI 10.1089/dna.1997.16.893
PMID 9260932
MeSH 3T3 Cells Amino Acid Sequence Animals Bacteriophage T7 / genetics Bacteriophage lambda / genetics* Blood Proteins / genetics Cloning, Molecular / methods* DNA, Complementary / genetics Genes, myc / genetics Genetic Vectors / genetics* Hemagglutinins, Viral / genetics Mice Molecular Sequence Data Plasmids / genetics Recombinant Fusion Proteins / biosynthesis*
IF 2.918
Times Cited 5
WOS Category GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
DNA material S-HA (pRc/CMV) (RDB02136) S-T7 (RDB02137) D-T7 (RDB02138) D-HA (RDB02139) S-Myc (RDB02140) D-Myc (RDB02141)