論文 - 詳細
| RRC ID | 29149 |
|---|---|
| 著者 | Kamimura Y, Tak YS, Sugino A, Araki H. |
| タイトル | Sld3, which interacts with Cdc45 (Sld4), functions for chromosomal DNA replication in Saccharomyces cerevisiae. |
| ジャーナル | EMBO J |
| Abstract |
Cdc45, which binds to the minichromosomal maintenance (Mcm) proteins, has a pivotal role in the initiation and elongation steps of chromosomal DNA replication in eukaryotes. Here we show that throughout the cell cycle in Saccharomyces cerevisiae, Cdc45 forms a complex with a novel factor, Sld3. Consistently, Sld3 and Cdc45 associate simultaneously with replication origins in the chromatin immunoprecipitation assay: both proteins associate with early-firing origins in G(1) phase and with late-firing origins in late S phase. Moreover, the origin associations of Sld3 and Cdc45 are mutually dependent. The temperature-sensitive sld3 mutation confers a defect in DNA replication at the restrictive temperature and reduces an interaction not only between Sld3 and Cdc45, but also between Cdc45 and Mcm2. These results suggest that the Sld3-Cdc45 complex associates with replication origins through Mcm proteins. At the restrictive temperature in sld3-5 cells, replication factor A, a single-strand DNA binding protein, does not associate with origins. Therefore, the origin association of Sld3-Cdc45 complex is prerequisite for origin unwinding in the initiation of DNA replication. |
| 巻・号 | 20(8) |
| ページ | 2097-107 |
| 公開日 | 2001-4-17 |
| DOI | 10.1093/emboj/20.8.2097 |
| PMID | 11296242 |
| PMC | PMC125422 |
| MeSH | Carrier Proteins / metabolism* Cell Cycle Proteins / genetics* Cell Cycle Proteins / metabolism* Chromosomes, Fungal / metabolism* DNA Replication* DNA, Fungal / biosynthesis* DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism* Gene Dosage Genes, Essential Genes, Fungal Nuclear Proteins / metabolism* Protein Binding Replication Origin Replication Protein A S Phase Saccharomyces cerevisiae / cytology Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae Proteins* |
| IF | 9.889 |
| 引用数 | 160 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pBS(SK+)SLD3 (RDB02944) |