RRC ID 29149
Author Kamimura Y, Tak YS, Sugino A, Araki H.
Title Sld3, which interacts with Cdc45 (Sld4), functions for chromosomal DNA replication in Saccharomyces cerevisiae.
Journal EMBO J
Abstract Cdc45, which binds to the minichromosomal maintenance (Mcm) proteins, has a pivotal role in the initiation and elongation steps of chromosomal DNA replication in eukaryotes. Here we show that throughout the cell cycle in Saccharomyces cerevisiae, Cdc45 forms a complex with a novel factor, Sld3. Consistently, Sld3 and Cdc45 associate simultaneously with replication origins in the chromatin immunoprecipitation assay: both proteins associate with early-firing origins in G(1) phase and with late-firing origins in late S phase. Moreover, the origin associations of Sld3 and Cdc45 are mutually dependent. The temperature-sensitive sld3 mutation confers a defect in DNA replication at the restrictive temperature and reduces an interaction not only between Sld3 and Cdc45, but also between Cdc45 and Mcm2. These results suggest that the Sld3-Cdc45 complex associates with replication origins through Mcm proteins. At the restrictive temperature in sld3-5 cells, replication factor A, a single-strand DNA binding protein, does not associate with origins. Therefore, the origin association of Sld3-Cdc45 complex is prerequisite for origin unwinding in the initiation of DNA replication.
Volume 20(8)
Pages 2097-107
Published 2001-4-17
DOI 10.1093/emboj/20.8.2097
PMID 11296242
PMC PMC125422
MeSH Carrier Proteins / metabolism* Cell Cycle Proteins / genetics* Cell Cycle Proteins / metabolism* Chromosomes, Fungal / metabolism* DNA Replication* DNA, Fungal / biosynthesis* DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism* Gene Dosage Genes, Essential Genes, Fungal Nuclear Proteins / metabolism* Protein Binding Replication Origin Replication Protein A S Phase Saccharomyces cerevisiae / cytology Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae Proteins*
IF 9.889
Times Cited 160
DNA material pBS(SK+)SLD3 (RDB02944)