| RRC ID |
29163
|
| Author |
Hirao K, Hata Y, Ide N, Takeuchi M, Irie M, Yao I, Deguchi M, Toyoda A, Sudhof TC, Takai Y.
|
| Title |
A novel multiple PDZ domain-containing molecule interacting with N-methyl-D-aspartate receptors and neuronal cell adhesion proteins.
|
| Journal |
J Biol Chem
|
| Abstract |
At synaptic junctions, pre- and postsynaptic membranes are connected by cell adhesion and have distinct structures for specialized functions. The presynaptic membranes have a machinery for fast neurotransmitter release, and the postsynaptic membranes have clusters of neurotransmitter receptors. The molecular mechanism of the assembly of synaptic junctions is not yet clear. Pioneering studies identified postsynaptic density (PSD)-95/SAP90 as a prototypic synaptic scaffolding protein to maintain the structure of synaptic junctions. PSD-95/SAP90 belongs to a family of membrane-associated guanylate kinases and binds N-methyl-D-aspartate receptors, potassium channels, and neuroligins through the PDZ domains and GKAP/SAPAP/DAP through the guanylate kinase (GK) domain. We performed here a yeast two-hybrid screening for SAPAP-interacting molecules and identified a novel protein that has an inverse structure of membrane-associated guanylate kinases with an NH2-terminal GK-like domain followed by two WW and five PDZ domains. It binds SAPAP through the GK-like domain and NMDA receptors and neuroligins through the PDZ domains. We named this protein S-SCAM (synaptic scaffolding molecule) because S-SCAM may assemble receptors and cell adhesion proteins at synaptic junctions.
|
| Volume |
273(33)
|
| Pages |
21105-10
|
| Published |
1998-8-14
|
| DOI |
10.1074/jbc.273.33.21105
|
| PII |
S0021-9258(18)48957-0
|
| PMID |
9694864
|
| MeSH |
Adaptor Proteins, Signal Transducing
Amino Acid Sequence
Animals
Base Sequence
COS Cells
Carrier Proteins / chemistry
Carrier Proteins / genetics
Carrier Proteins / metabolism*
Cell Adhesion Molecules, Neuronal / metabolism*
Cell Line
Cell Membrane / metabolism
DNA, Complementary
Disks Large Homolog 4 Protein
Guanylate Kinases
Humans
Intracellular Signaling Peptides and Proteins
Membrane Proteins
Molecular Sequence Data
Nerve Tissue Proteins / metabolism
Precipitin Tests
Protein Binding
Rats
Receptors, N-Methyl-D-Aspartate / metabolism*
SAP90-PSD95 Associated Proteins
Saccharomyces cerevisiae / genetics
|
| IF |
4.238
|
| Times Cited |
229
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
S-SCAM (p3205-54) (RDB03435) |
