論文 - 詳細
| RRC ID | 29163 |
|---|---|
| 著者 | Hirao K, Hata Y, Ide N, Takeuchi M, Irie M, Yao I, Deguchi M, Toyoda A, Sudhof TC, Takai Y. |
| タイトル | A novel multiple PDZ domain-containing molecule interacting with N-methyl-D-aspartate receptors and neuronal cell adhesion proteins. |
| ジャーナル | J Biol Chem |
| Abstract |
At synaptic junctions, pre- and postsynaptic membranes are connected by cell adhesion and have distinct structures for specialized functions. The presynaptic membranes have a machinery for fast neurotransmitter release, and the postsynaptic membranes have clusters of neurotransmitter receptors. The molecular mechanism of the assembly of synaptic junctions is not yet clear. Pioneering studies identified postsynaptic density (PSD)-95/SAP90 as a prototypic synaptic scaffolding protein to maintain the structure of synaptic junctions. PSD-95/SAP90 belongs to a family of membrane-associated guanylate kinases and binds N-methyl-D-aspartate receptors, potassium channels, and neuroligins through the PDZ domains and GKAP/SAPAP/DAP through the guanylate kinase (GK) domain. We performed here a yeast two-hybrid screening for SAPAP-interacting molecules and identified a novel protein that has an inverse structure of membrane-associated guanylate kinases with an NH2-terminal GK-like domain followed by two WW and five PDZ domains. It binds SAPAP through the GK-like domain and NMDA receptors and neuroligins through the PDZ domains. We named this protein S-SCAM (synaptic scaffolding molecule) because S-SCAM may assemble receptors and cell adhesion proteins at synaptic junctions. |
| 巻・号 | 273(33) |
| ページ | 21105-10 |
| 公開日 | 1998-8-14 |
| DOI | 10.1074/jbc.273.33.21105 |
| PII | S0021-9258(18)48957-0 |
| PMID | 9694864 |
| MeSH | Adaptor Proteins, Signal Transducing Amino Acid Sequence Animals Base Sequence COS Cells Carrier Proteins / chemistry Carrier Proteins / genetics Carrier Proteins / metabolism* Cell Adhesion Molecules, Neuronal / metabolism* Cell Line Cell Membrane / metabolism DNA, Complementary Disks Large Homolog 4 Protein Guanylate Kinases Humans Intracellular Signaling Peptides and Proteins Membrane Proteins Molecular Sequence Data Nerve Tissue Proteins / metabolism Precipitin Tests Protein Binding Rats Receptors, N-Methyl-D-Aspartate / metabolism* SAP90-PSD95 Associated Proteins Saccharomyces cerevisiae / genetics |
| IF | 4.238 |
| 引用数 | 229 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 14 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | S-SCAM (p3205-54) (RDB03435) |