RRC ID 29165
Author Fukasawa KM, Fukasawa K, Kanai M, Fujii S, Harada M.
Title Molecular cloning and expression of rat liver aminopeptidase B.
Journal J. Biol. Chem.
Abstract We isolated, by immunological screening of a Uni-ZAP XR cDNA library constructed from rat liver mRNAs, a cDNA clone with 2212 base pairs encoding aminopeptidase B (EC 3.4.11.6). The open reading frame encodes a 649-amino acid protein with a theoretical molecular mass of 72,545 Da and bears the consensus sequence of the zinc metalloexopeptidases, indicating that the enzyme belongs to this family, which includes aminopeptidase A, aminopeptidase N, and leukotriene-A4 hydrolase. Escherichia coli SOLR cells infected with the pBluescript phagemid excised from the Uni-ZAP XR vector containing the aminopeptidase B cDNA had a high L-arginyl-beta-naphthylamidase activity. The recombinant protein was purified to homogeneity from the recombinant E. coli extracts. The enzyme had Cl--dependent aminopeptidase activity specifically restricted to the Arg and Lys derivatives and contained 1 mol of zinc per mol of the enzyme.
Volume 271(48)
Pages 30731-5
Published 1996-11-29
PMID 8940051
MeSH Amino Acid Sequence Aminopeptidases / genetics* Animals Base Sequence Cloning, Molecular DNA, Complementary / genetics Epoxide Hydrolases / chemistry Gene Expression Humans Liver / enzymology Metalloproteins / genetics Molecular Sequence Data Rats Sequence Alignment Zinc
IF 4.011
Times Cited 34
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
DNA material pBlueRApB (RDB07717) pGEXRApB (RDB07718)