Reference - Detail
| RRC ID | 29165 |
|---|---|
| Author | Fukasawa KM, Fukasawa K, Kanai M, Fujii S, Harada M. |
| Title | Molecular cloning and expression of rat liver aminopeptidase B. |
| Journal | J Biol Chem |
| Abstract |
We isolated, by immunological screening of a Uni-ZAP XR cDNA library constructed from rat liver mRNAs, a cDNA clone with 2212 base pairs encoding aminopeptidase B (EC 3.4.11.6). The open reading frame encodes a 649-amino acid protein with a theoretical molecular mass of 72,545 Da and bears the consensus sequence of the zinc metalloexopeptidases, indicating that the enzyme belongs to this family, which includes aminopeptidase A, aminopeptidase N, and leukotriene-A4 hydrolase. Escherichia coli SOLR cells infected with the pBluescript phagemid excised from the Uni-ZAP XR vector containing the aminopeptidase B cDNA had a high L-arginyl-beta-naphthylamidase activity. The recombinant protein was purified to homogeneity from the recombinant E. coli extracts. The enzyme had Cl--dependent aminopeptidase activity specifically restricted to the Arg and Lys derivatives and contained 1 mol of zinc per mol of the enzyme. |
| Volume | 271(48) |
| Pages | 30731-5 |
| Published | 1996-11-29 |
| DOI | 10.1074/jbc.271.48.30731 |
| PII | S0021-9258(19)79124-8 |
| PMID | 8940051 |
| MeSH | Amino Acid Sequence Aminopeptidases / genetics* Animals Base Sequence Cloning, Molecular DNA, Complementary / genetics Epoxide Hydrolases / chemistry Gene Expression Humans Liver / enzymology Metalloproteins / genetics Molecular Sequence Data Rats Sequence Alignment Zinc |
| IF | 4.238 |
| Times Cited | 35 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 8 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pBlueRApB (RDB07717) pGEXRApB (RDB07718) |