| RRC ID |
29165
|
| 著者 |
Fukasawa KM, Fukasawa K, Kanai M, Fujii S, Harada M.
|
| タイトル |
Molecular cloning and expression of rat liver aminopeptidase B.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
We isolated, by immunological screening of a Uni-ZAP XR cDNA library constructed from rat liver mRNAs, a cDNA clone with 2212 base pairs encoding aminopeptidase B (EC 3.4.11.6). The open reading frame encodes a 649-amino acid protein with a theoretical molecular mass of 72,545 Da and bears the consensus sequence of the zinc metalloexopeptidases, indicating that the enzyme belongs to this family, which includes aminopeptidase A, aminopeptidase N, and leukotriene-A4 hydrolase. Escherichia coli SOLR cells infected with the pBluescript phagemid excised from the Uni-ZAP XR vector containing the aminopeptidase B cDNA had a high L-arginyl-beta-naphthylamidase activity. The recombinant protein was purified to homogeneity from the recombinant E. coli extracts. The enzyme had Cl--dependent aminopeptidase activity specifically restricted to the Arg and Lys derivatives and contained 1 mol of zinc per mol of the enzyme.
|
| 巻・号 |
271(48)
|
| ページ |
30731-5
|
| 公開日 |
1996-11-29
|
| DOI |
10.1074/jbc.271.48.30731
|
| PII |
S0021-9258(19)79124-8
|
| PMID |
8940051
|
| MeSH |
Amino Acid Sequence
Aminopeptidases / genetics*
Animals
Base Sequence
Cloning, Molecular
DNA, Complementary / genetics
Epoxide Hydrolases / chemistry
Gene Expression
Humans
Liver / enzymology
Metalloproteins / genetics
Molecular Sequence Data
Rats
Sequence Alignment
Zinc
|
| IF |
4.238
|
| 引用数 |
35
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
pBlueRApB (RDB07717)
pGEXRApB (RDB07718) |
