| RRC ID |
29168
|
| Author |
Hiroi T, Watabe S, Takimoto K, Yago N, Yamamoto Y, Takahashi SY.
|
| Title |
The cDNA sequence encoding bovine SP-22, a new defence system against reactive oxygen species in mitochondria.
|
| Journal |
DNA Seq
|
| Abstract |
We have isolated cDNA clones coding SP-22, an antioxidant protein in mitochondria, from a bovine adrenal medulla cDNA library constructed with (lambda)gt11. The largest clone contained the entire coding sequence for mature SP-22. Since the isolated cDNA clones lacked 5'- and 3'-ends, we determined the sequences of both ends by the "Rapid Amplification of cDNA Ends (RACE)" tecnique. The deduced amino acid sequence of the mature protein region was the same as that determined by protein sequencing. Since SP-22 had a mitochondrial targetting signal, its mitochondrial localization was confirmed.
|
| Volume |
6(4)
|
| Pages |
239-42
|
| Published |
1996-1-1
|
| DOI |
10.3109/10425179609008449
|
| PMID |
8912927
|
| MeSH |
Adrenal Medulla / metabolism*
Amino Acid Sequence
Animals
Antioxidants
Base Sequence
Cattle
DNA Primers
DNA, Complementary
DNA, Mitochondrial / chemistry
Membrane Proteins / biosynthesis*
Membrane Proteins / chemistry
Mitochondria / metabolism*
Molecular Sequence Data
Peroxidases*
Peroxiredoxins
Polymerase Chain Reaction
Reactive Oxygen Species
Restriction Mapping
Sequence Homology, Amino Acid
|
| Times Cited |
11
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
|
| Resource |
| DNA material |
pSP22-4 (RDB01396) |
