| RRC ID |
29177
|
| 著者 |
Tamechika I, Itakura M, Saruta Y, Furukawa M, Kato A, Tachibana S, Hirose S.
|
| タイトル |
Accelerated evolution in inhibitor domains of porcine elafin family members.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
Through the analysis of the porcine gene encoding the elastase inhibitor elafin, we demonstrated that there are at least three closely related members of the elafin family, and their genes have arisen by accelerated evolution. A porcine genomic DNA library was screened with a previously cloned human elafin cDNA probe, and several positive clones were obtained that can be distinguished by a combination of restriction enzymes. Sequence analysis of these clones revealed the presence of three homologous members whose genes, all consisting of three exons and two introns, are almost identical except the exon 2 sequences encoding the inhibitor domain called "WAP motif"; the intron sequences are related to each other with sequence similarities of 93-98%, whereas the exon 2 sequences exhibited only 60-77% similarities among the three members. The extreme divergence in the exon 2 sequences compared to the highly conserved intron sequences may be generated by accelerated mutations confined in a short stretch of the genes following recent duplication events of a single ancestral gene. An RNase protection assay indicated that the messages of the elafin family members are abundantly expressed in the trachea and intestine, suggesting that the most likely selective forces for the accelerated evolution are extrinsic proteinases produced by invasive microorganisms.
|
| 巻・号 |
271(12)
|
| ページ |
7012-8
|
| 公開日 |
1996-3-22
|
| DOI |
10.1074/jbc.271.12.7012
|
| PII |
S0021-9258(17)45065-4
|
| PMID |
8636131
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Biological Evolution*
Cloning, Molecular
DNA
Exons
Humans
Introns
Molecular Sequence Data
Mutation
Proteinase Inhibitory Proteins, Secretory
Proteins / genetics*
Repetitive Sequences, Nucleic Acid
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Serine Proteinase Inhibitors / genetics*
Swine
|
| IF |
4.238
|
| 引用数 |
42
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
Porcine gene for elafin (RDB01812)
Porcine gene for SPA1 (RDB01813)
Porcine gene for elafin homolog (RDB01814) |
