RRC ID |
29191
|
著者 |
Taira M, Kudoh J, Minoshima S, Iizasa T, Shimada H, Shimizu Y, Tatibana M, Shimizu N.
|
タイトル |
Localization of human phosphoribosylpyrophosphate synthetase subunit I and II genes (PRPS1 and PRPS2) to different regions of the X chromosome and assignment of two PRPS1-related genes to autosomes.
|
ジャーナル |
Somat Cell Mol Genet
|
Abstract |
Complementary DNA clones for phosphoribosylpyrophosphate synthetase subunits I and II (PRS I and PRS II) were used to determine the chromosomal localization of the corresponding human genes. Southern blot analysis of genomic DNAs isolated from human placenta and a panel of human-mouse somatic cell hybrids revealed that the rat PRS I cDNA probe detected at least five human specific DNA segments (23, 20, 14.5, 6.7, and 4.3 kb) in BamHI digests. The 23-, 14.5-, and 6.7-kb DNA segments were detected only if the hybrids contained human chromosome X or translocation chromosome 7p+ (7qter greater than 7p22::Xq21 greater than Xqter), indicating the location of these segments to Xq21-qter (PRPS1). The 20- and 4.3-kb DNA segments did not cosegregate with the other three segments, and spot blot hybridization analysis using flow-sorted human chromosomes indicated that these are the PRPS1-related genes (PRPS1L1 and PRPS1L2) and could be assigned to chromosomes 7 and 9, respectively. The human-specific PRS II cDNA probe revealed a BamHI DNA segment (17 kb), which segregated condordantly with the X chromosome but not with the PRPS1 gene. We surmise that the gene for PRS II (PRPS2) is located at a different region of the X chromosome, namely Xpter-a21.
|
巻・号 |
15(1)
|
ページ |
29-37
|
公開日 |
1989-1-1
|
DOI |
10.1007/BF01534667
|
PMID |
2536962
|
MeSH |
Animals
Blotting, Southern
Cell Line
Chromosome Mapping*
Chromosomes, Human, Pair 7*
Chromosomes, Human, Pair 9*
DNA
DNA Probes
Genes*
Humans
Hybrid Cells
Karyotyping / methods
Mice
Phosphotransferases / genetics*
Rats
Ribose-Phosphate Pyrophosphokinase / genetics*
X Chromosome*
|
引用数 |
43
|
WOS 分野
|
GENETICS & HEREDITY
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
リソース情報 |
遺伝子材料 |
pPRS I-24 (RDB01370)
pPRS II-54 (RDB01371) |