| RRC ID |
29198
|
| Author |
Choi YL, Nishida T, Kawamukai M, Utsumi R, Sakai H, Komano T.
|
| Title |
Cloning and sequencing of an Escherichia coli gene, nlp, highly homologous to the ner genes of bacteriophages Mu and D108.
|
| Journal |
J Bacteriol
|
| Abstract |
An nlp (Ner-like protein) gene was isolated from Escherichia coli. The nucleotide sequence of a 1,342-base-pair chromosomal DNA fragment containing the nlp gene was analyzed. It contained two open reading frames; one encoded 91 amino acid residues with an Mr of 10,361, and the other (ORFX) encoded 131 amino acid residues of the carboxyl-terminal region of a truncated polypeptide. The amino acid sequence deduced from the DNA sequence of nlp was highly homologous (62 to 63%) to the Ner proteins of bacteriophages Mu and D108. The amino-terminal region of Nlp deduced from the complete open reading frame contained a presumed DNA-binding region. The nlp gene was located at 69.3 min on the E. coli genetic map.
|
| Volume |
171(9)
|
| Pages |
5222-5
|
| Published |
1989-9-1
|
| DOI |
10.1128/jb.171.9.5222-5225.1989
|
| PMID |
2670911
|
| PMC |
PMC210348
|
| MeSH |
Amino Acid Sequence
Base Sequence
Cloning, Molecular
Coliphages / genetics*
DNA-Binding Proteins / genetics*
Escherichia coli / genetics*
Genes*
Genes, Bacterial*
Genes, Viral*
Molecular Sequence Data
Repressor Proteins / genetics*
Restriction Mapping
Sequence Homology, Nucleic Acid
Transcription Factors / genetics*
Viral Proteins / genetics*
Viral Regulatory and Accessory Proteins
|
| IF |
3.006
|
| Times Cited |
18
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| DNA material |
pKA3 (RDB01546)
pKA7 (RDB01547) |
