論文 - 詳細
| RRC ID | 29199 |
|---|---|
| 著者 | Noguchi N, Shishido K, Ando T, Kono M. |
| タイトル | Construction and propagation of deletion derivatives of staphylococcal tetracycline-resistance plasmid pTP-5 in Bacillus subtilis. |
| ジャーナル | Gene |
| Abstract |
Tetracycline-resistance (TCr) plasmid pTP-5 (4.46 kb) from Staphylococcus aureus was cleaved with HindIII into three fragments: A (2.35 kb), B (1.57 kb) and C (0.54 kb). A deletion plasmid (3.92 kb) lacking HindIII-C fragment was obtained, and was designated pNS1. This plasmid retained the TCr phenotype and the ability to replicate autonomously in Bacillus subtilis. A restriction endonuclease cleavage map of pNS1 was constructed. Attempts to construct smaller plasmids by digesting pNS1 with BAL31 nuclease led to production of a set of deletion derivatives whose molecular sizes range from 3.75 to 2.77 kb. Through analyses of these derivatives, the regions essential for autonomous replication and expression of TCr were deduced. |
| 巻・号 | 21(1-2) |
| ページ | 105-10 |
| 公開日 | 1983-1-1 |
| DOI | 10.1016/0378-1119(83)90152-x |
| PII | 0378-1119(83)90152-X |
| PMID | 6301940 |
| MeSH | Bacillus subtilis / drug effects Bacillus subtilis / genetics* Chromosome Deletion* Chromosomes, Bacterial DNA Restriction Enzymes Drug Resistance, Microbial Phenotype Plasmids* Staphylococcus aureus / drug effects Staphylococcus aureus / genetics* Tetracycline / pharmacology* |
| IF | 2.984 |
| 引用数 | 24 |
| WOS 分野 | GENETICS & HEREDITY |
| リソース情報 | |
| 遺伝子材料 | Plasmid pTP5 (pTZ5) (RDB01806) |