RRC ID 292
Author Norga KK, Gurganus MC, Dilda CL, Yamamoto A, Lyman RF, Patel PH, Rubin GM, Hoskins RA, Mackay TF, Bellen HJ.
Title Quantitative analysis of bristle number in Drosophila mutants identifies genes involved in neural development.
Journal Curr Biol
Abstract BACKGROUND:The identification of the function of all genes that contribute to specific biological processes and complex traits is one of the major challenges in the postgenomic era. One approach is to employ forward genetic screens in genetically tractable model organisms. In Drosophila melanogaster, P element-mediated insertional mutagenesis is a versatile tool for the dissection of molecular pathways, and there is an ongoing effort to tag every gene with a P element insertion. However, the vast majority of P element insertion lines are viable and fertile as homozygotes and do not exhibit obvious phenotypic defects, perhaps because of the tendency for P elements to insert 5' of transcription units. Quantitative genetic analysis of subtle effects of P element mutations that have been induced in an isogenic background may be a highly efficient method for functional genome annotation.
RESULTS:Here, we have tested the efficacy of this strategy by assessing the extent to which screening for quantitative effects of P elements on sensory bristle number can identify genes affecting neural development. We find that such quantitative screens uncover an unusually large number of genes that are known to function in neural development, as well as genes with yet uncharacterized effects on neural development, and novel loci.
CONCLUSIONS:Our findings establish the use of quantitative trait analysis for functional genome annotation through forward genetics. Similar analyses of quantitative effects of P element insertions will facilitate our understanding of the genes affecting many other complex traits in Drosophila.
Volume 13(16)
Pages 1388-96
Published 2003-8-19
DOI 10.1016/s0960-9822(03)00546-3
PII S0960-9822(03)00546-3
PMID 12932322
MeSH Animals Drosophila / anatomy & histology Drosophila / genetics* Drosophila / growth & development* Gene Expression Regulation, Developmental Genes, Insect* Mutation Peripheral Nerves / growth & development
IF 9.601
Times Cited 98