| RRC ID |
29208
|
| Author |
Noguchi N, Sasatsu M, Kono M.
|
| Title |
Genetic mapping in Bacillus subtilis 168 of the aadK gene which encodes aminoglycoside 6-adenylyltransferase.
|
| Journal |
FEMS Microbiol Lett
|
| Abstract |
Bacillus subtilis 168 has an aadK gene, which encodes aminoglycoside 6-adenylyltransferase, a streptomycin-modifying enzyme, on its chromosome. To characterize the aadK gene, we constructed a B. subtilis 168 strain that carried the chloramphenicol resistance gene near the aadK on the chromosome and an aadK deletion mutant using an integration technique. The aadK gene was mapped between azlB and pheA on the chromosome of B. subtilis 168. The aadK deletion mutant was slightly more susceptible to streptomycin than the original strain. The result indicates that the aadK gene contributes low-level resistance to streptomycin in B. subtilis 168.
|
| Volume |
114(1)
|
| Pages |
47-52
|
| Published |
1993-11-15
|
| DOI |
10.1016/0378-1097(93)90140-w
|
| PII |
0378-1097(93)90140-W
|
| PMID |
8293959
|
| MeSH |
Bacillus subtilis / drug effects
Bacillus subtilis / enzymology
Bacillus subtilis / genetics*
Chromosome Mapping
Chromosomes, Bacterial
Cloning, Molecular
Crosses, Genetic
Drug Resistance, Microbial
Genes, Bacterial / genetics*
Microbial Sensitivity Tests
Mutation
Nucleotidyltransferases / genetics*
Plasmids
Streptomycin / pharmacology*
Transduction, Genetic
|
| IF |
1.987
|
| Times Cited |
9
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| DNA material |
pTZ331 (RDB01804) |
