論文 - 詳細
| RRC ID | 29220 |
|---|---|
| 著者 | Koga M, Matsuda M, Kawamura T, Sogo T, Shigeno A, Nishida E, Ebisuya M. |
| タイトル | Foxd1 is a mediator and indicator of the cell reprogramming process. |
| ジャーナル | Nat Commun |
| Abstract |
It remains unclear how changes in gene expression profiles that establish a pluripotent state are induced during cell reprogramming. Here we identify two forkhead box transcription factors, Foxd1 and Foxo1, as mediators of gene expression programme changes during reprogramming. Knockdown of Foxd1 or Foxo1 reduces the number of iPSCs, and the double knockdown further reduces it. Knockout of Foxd1 inhibits downstream transcriptional events, including the expression of Dax1, a component of the autoregulatory network for maintaining pluripotency. Interestingly, the expression level of Foxd1 is transiently increased in a small population of cells in the middle stage of reprogramming. The transient Foxd1 upregulation in this stage is correlated with a future cell fate as iPSCs. Fate mapping analyses further reveal that >95% of iPSC colonies are derived from the Foxd1-positive cells. Thus, Foxd1 is a mediator and indicator of successful progression of reprogramming. |
| 巻・号 | 5 |
| ページ | 3197 |
| 公開日 | 2014-1-1 |
| DOI | 10.1038/ncomms4197 |
| PII | ncomms4197 |
| PMID | 24496101 |
| MeSH | Animals Cellular Reprogramming* Forkhead Box Protein O1 Forkhead Transcription Factors / genetics Forkhead Transcription Factors / metabolism* Gene Expression Regulation* Gene Knockdown Techniques HEK293 Cells Humans Mice, Inbred C57BL Mice, Inbred ICR |
| IF | 12.121 |
| 引用数 | 35 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC02290 |
| ヒト・動物細胞 | iPS-MEF-Ng-20D-17(APS0001) |